Pepsin egg white hydrolysate modulates gut microbiota in Zucker obese rats

There is limited information that relates the intake of food-derived bioactive peptides and the gut microbiota. We have previously described a pepsin hydrolysate of egg white (EWH) that ameliorates fat accumulation and dyslipidemia, while reducing oxidative stress and inflammation markers in obese Z...

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Bibliographic Details
Published in:Food & function 2017-01, Vol.8 (1), p.437-443
Main Authors: Requena, Teresa, Miguel, Marta, Garcés-Rimón, Marta, Martínez-Cuesta, M. Carmen, López-Fandiño, Rosina, Peláez, Carmen
Format: Article
Language:English
Subjects:
Animals
Bacteria - classification
Bacteria - genetics
Bacteria - isolation & purification
Bacteria - metabolism
Clostridium leptum
Egg Proteins - chemistry
Egg Proteins - metabolism
Egg White - chemistry
Enterococcus
Fatty Acids, Volatile - metabolism
Gastrointestinal Microbiome
Humans
Lactobacillus
Male
Obesity - diet therapy
Obesity - metabolism
Obesity - microbiology
Oxidative Stress
Pepsin A - chemistry
Peptides - chemistry
Peptides - metabolism
Protein Hydrolysates - chemistry
Protein Hydrolysates - metabolism
Rats
Rats, Zucker
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Summary:There is limited information that relates the intake of food-derived bioactive peptides and the gut microbiota. We have previously described a pepsin hydrolysate of egg white (EWH) that ameliorates fat accumulation and dyslipidemia, while reducing oxidative stress and inflammation markers in obese Zucker rats. The aim of this study was to associate the beneficial effects of EWH with gut microbiota changes in these animals. Obese Zucker rats received daily 750 mg kg −1 EWH in drinking water for 12 weeks and faeces were analysed for microbial composition and metabolic compounds in comparison with Zucker lean rats and obese controls. EWH supplementation modulated the microbiological characteristics of the obese rats to values similar to those of the lean rats. Specifically, counts of total bacteria, Lactobacillus / Enterococcus and Clostridium leptum in EWH fed obese Zucker rats were more similar to the lean rats than to the obese controls. Besides, feeding the obese Zucker rats with EWH reduced ( P < 0.05) the faecal concentration of lactic acid. The physiological benefits of EWH in the improvement of obesity associated complications of Zucker rats could be associated with a more lean-like gut microbiota and a tendency to diminish total short-chain fatty acids (SCFA) production and associated obesity complications. The results warrant the use of pepsin egg white hydrolysate as a bioactive food ingredient. Pepsin egg white hydrolysate favours lean-associated microbiota and microbial metabolism.
ISSN:2042-6496
2042-650X
DOI:10.1039/c6fo01571a