Amorphous carbon dots with high two-photon fluorescence for cellular imaging passivated by hyperbranched poly(amino amine)Electronic supplementary information (ESI) available: Fig. S1-S3, Table S1. See DOI: 10.1039/c4tb01643b

Amorphous carbon dots (C-Dots) with high two-photon fluorescence were prepared by using citric acid (CA) as the carbon source and hyperbranched poly(amino amine) (HPAA) as the surface passivation agent through a facile hydrothermal approach. The C-Dots with an average diameter about 10 nm were readi...

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Main Authors: Tong, Gangsheng, Wang, Jingxia, Wang, Ruibin, Guo, Xinqiu, He, Lin, Qiu, Feng, Wang, Ge, Zhu, Bangshang, Zhu, Xinyuan, Liu, Tao
Format: Article
Language:English
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Summary:Amorphous carbon dots (C-Dots) with high two-photon fluorescence were prepared by using citric acid (CA) as the carbon source and hyperbranched poly(amino amine) (HPAA) as the surface passivation agent through a facile hydrothermal approach. The C-Dots with an average diameter about 10 nm were readily dispersed in water. They exhibited excellent fluorescence properties and excitation-dependent fluorescence behavior with the corresponding quantum yield (QY) of 17.1% in aqueous solution. Interestingly, the C-Dots emitted bright fluorescence even in the solid state with a QY of 16.3%, which is the highest value obtained for carbon-based nanomaterials. Using the MTT assay, the C-Dots showed low cytotoxicity against L929 normal cells. Furthermore, they were easily internalized by HeLa cells and presented high quality one- and two-photon cellular imaging, suggesting significant potential for application in biological imaging. Amorphous carbon dots with high two-photon fluorescence in aqueous solution and solid state were prepared through a facile hydrothermal approach.
ISSN:2050-750X
2050-7518
DOI:10.1039/c4tb01643b