Self-organization of the bacterial cell-division protein FtsZ in confined environmentsElectronic supplementary information (ESI) available: Microfluidic chip fabrication and design are shown in Fig. S1 and S2; effects of surfactant and oil on bulk emulsion FtsZ bundles are shown in Fig. S3 and S4; Fig. S5 shows FRAP of FtsZ bundles inside droplets at different crowder concentrations; Fig. S6 shows distribution of FtsZ bundles within spherical and disc-shaped droplets at 150 g L−1 Ficoll. See DOI

We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent, FtsZ, an...

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Main Authors: Mellouli, Sonia, Monterroso, Begoña, Vutukuri, Hanumantha Rao, Brinke, Esra te, Chokkalingam, Venkatachalam, Rivas, Germán, Huck, Wilhelm T. S
Format: Article
Language:English
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Summary:We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent, FtsZ, and GTP. FtsZ bundles confined in droplets were dynamic, and their distribution depended on the intrinsic properties of the system and restrictions imposed by the spatial boundaries. We report a microfluidic approach to generate aqueous droplets in oil of different dimensionality, stabilized by a lipid monolayer, to systematically probe the polymerization of bacterial cell-division protein FtsZ into fibrous networks as a function of the concentrations of crowding agent and FtsZ.
ISSN:1744-683X
1744-6848
DOI:10.1039/c3sm51163d