SWATH‐MS and MRM: Quantification of Ras‐related proteins in HIV‐1 infected and methamphetamine‐exposed human monocyte‐derived macrophages (hMDM)

HIV‐1 infection of macrophages is a multistep and multifactorial process that has been shown to be enhanced by exposure to methamphetamine (Meth). In this study, we sought to identify the underlying mechanisms of this effect by quantifying the effect of Meth on the proteome of HIV‐1‐infected macroph...

Full description

Saved in:
Bibliographic Details
Published in:Proteomics (Weinheim) 2021-08, Vol.21 (15), p.e2100005-n/a
Main Authors: Macur, Katarzyna, Zieschang, Sarah, Lei, Shulei, Morsey, Brenda, Jaquet, Spencer, Belshan, Michael, Fox, Howard S., Ciborowski, Pawel
Format: Article
Language:English
Subjects:
Actin
Exposure
HIV
HIV Infections
HIV-1
Homeostasis
Human immunodeficiency virus
Humans
Infections
Macrophages
Mass spectra
Methamphetamine
Methamphetamine - pharmacology
Monocytes
MRM
Proteins
Proteome
Proteomes
Rab proteins
SWATH‐MS
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:HIV‐1 infection of macrophages is a multistep and multifactorial process that has been shown to be enhanced by exposure to methamphetamine (Meth). In this study, we sought to identify the underlying mechanisms of this effect by quantifying the effect of Meth on the proteome of HIV‐1‐infected macrophages using sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH‐MS) approach. The analyses identified several members of the Rab family of proteins as being dysregulated by Meth treatment, which was confirmed by bioinformatic analyses that indicated substantial alteration of vesicular transport pathways. Validation of the SWATH‐MS was performed using an MRM based approach, which confirmed that Meth exposure affects expression of the Rab proteins. However, the pattern of expression changes were highly dynamic, and displayed high donor‐to‐donor variability. Surprisingly a similar phenomenon was observed for Actin. Our results demonstrate that Meth affects vesicular transport pathways, suggesting a possible molecular mechanism underlying its effect on HIV infection hMDM and a potential broader effect of Meth on cellular homeostasis.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.202100005