Dually innervated dendritic spines develop in the absence of excitatory activity and resist structural plasticity through tonic inhibitory crosstalk

Dendritic spines can be directly connected to both inhibitory and excitatory presynaptic terminals, resulting in nanometer-scale proximity of opposing synaptic functions. While dually innervated spines (DiSs) are observed throughout the central nervous system, their developmental timeline and functi...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 2022-11, Vol.111 (3), p.362-371.e6
Main Authors: Kleinjan, Mason S., Buchta, William C., Ogelman, Roberto, Hwang, In-Wook, Kuwajima, Masaaki, Hubbard, Dusten D., Kareemo, Dean J., Prikhodko, Olga, Olah, Samantha L., Wulschner, Luis E. Gomez, Abraham, Wickliffe C., Franco, Santos J., Harris, Kristen M., Oh, Won Chan, Kennedy, Matthew J.
Format: Article
Language:English
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Summary:Dendritic spines can be directly connected to both inhibitory and excitatory presynaptic terminals, resulting in nanometer-scale proximity of opposing synaptic functions. While dually innervated spines (DiSs) are observed throughout the central nervous system, their developmental timeline and functional properties remain uncharacterized. Here we used a combination of serial section electron microscopy, live imaging and local synapse activity manipulations to investigate DiSs development and function in rodent hippocampus. Dual innervation occurred early in development, even on spines where the excitatory input was locally silenced. Synaptic NMDA receptor currents were selectively reduced at DiSs through tonic GABA B receptor signaling. Accordingly, spine enlargement normally associated with long-term potentiation on singly innervated spines (SiSs) was blocked at DiSs. Silencing somatostatin interneurons or pharmacologically blocking GABA B Rs restored NMDA receptor function and structural plasticity to levels comparable to neighboring SiSs. Thus, hippocampal DiSs are stable structures where function and plasticity are potently regulated by nanometer-scale GABAergic signaling. Kleinjan et al. demonstrate formation of hippocampal dually innervated spines (DiSs) occurs early in development and does not require excitatory input. NMDA receptor function and structural plasticity are impaired at DiSs. These effects are mediated through tonic GABA B receptor signaling and may contribute to long-term DiS stability.
ISSN:0896-6273
1097-4199
DOI:10.1016/j.neuron.2022.11.002