Adipose Cells Induce Escape from an Engineered Human Breast Microtumor Independently of their Obesity Status

Introduction Obesity is associated with increased breast cancer incidence, recurrence, and mortality. Adipocytes and adipose-derived stem cells (ASCs), two resident cell types in adipose tissue, accelerate the early stages of breast cancer progression. It remains unclear whether obesity plays a role...

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Published in:Cellular and molecular bioengineering 2023-02, Vol.16 (1), p.23-39
Main Authors: Dance, Yoseph W., Obenreder, Mackenzie C., Seibel, Alex J., Meshulam, Tova, Ogony, Joshua W., Lahiri, Nikhil, Pacheco-Spann, Laura, Radisky, Derek C., Layne, Matthew D., Farmer, Stephen R., Nelson, Celeste M., Tien, Joe
Format: Article
Language:English
Subjects:
Adipocytes
Adipose tissue
Biological and Medical Physics
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical Engineering/Biotechnology
Biophysics
Body fat
Breast cancer
Cell Biology
Cell culture
Engineering
Obesity
Original
Original Article
Stem cells
Stroma
Tissue engineering
Tumors
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Summary:Introduction Obesity is associated with increased breast cancer incidence, recurrence, and mortality. Adipocytes and adipose-derived stem cells (ASCs), two resident cell types in adipose tissue, accelerate the early stages of breast cancer progression. It remains unclear whether obesity plays a role in the subsequent escape of malignant breast cancer cells into the local circulation. Methods We engineered models of human breast tumors with adipose stroma that exhibited different obesity-specific alterations. We used these models to assess the invasion and escape of breast cancer cells into an empty, blind-ended cavity (as a mimic of a lymphatic vessel) for up to sixteen days. Results Lean and obese donor-derived adipose stroma hastened escape to similar extents. Moreover, a hypertrophic adipose stroma did not affect the rate of adipose-induced escape. When admixed directly into the model tumors, lean and obese donor-derived ASCs hastened escape similarly. Conclusions This study demonstrates that the presence of adipose cells, independently of the obesity status of the adipose tissue donor, hastens the escape of human breast cancer cells in multiple models of obesity-associated breast cancer.
ISSN:1865-5025
1865-5033
DOI:10.1007/s12195-022-00750-y