Rapid point of care testing for four bacterial sexually transmitted infections using the portable isothermal loop-mediated nucleic acid amplification eazyplex platform

Purpose To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex testing platform for bacterial sexually transmitted infections (STI) among men who have sex with men (MSM). Methods 272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both the eazyple...

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Bibliographic Details
Published in:Infection 2023-08, Vol.51 (4), p.1141-1145
Main Authors: Esitgen Germaner, Ece T., Wassill, Lars, Dichtl, Karl, Roider, Julia, Seybold, Ulrich
Format: Article
Language:English
Subjects:
Bacteria
Brief Report
Chlamydia Infections - diagnosis
Chlamydia trachomatis - genetics
Diagnostic systems
Family Medicine
Gays & lesbians
General Practice
Gonorrhea - diagnosis
Gonorrhea - microbiology
Homosexuality, Male
Humans
Infectious Diseases
Internal Medicine
Male
Medicine
Medicine & Public Health
Mens health
Microbiology
Neisseria gonorrhoeae - genetics
Nucleic acids
Pharynx
Point-of-Care Testing
Sensitivity analysis
Sexual and Gender Minorities
Sexually transmitted diseases
STD
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Summary:Purpose To analyze sensitivity and specificity of the rapid point-of-care (POC) eazyplex testing platform for bacterial sexually transmitted infections (STI) among men who have sex with men (MSM). Methods 272 anal, urethral, and pharyngeal swabs collected from 153 MSM were tested by both the eazyplex platform and an in-house PCR or culture in the university microbiology reference laboratory. Results Compared to the reference diagnostic method, the overall sensitivity/specificity of eazyplex was 88%/98% for N. gonorrhoeae , 82%/100% for C. trachomatis , 70%/ > 99% for U. urealyticum, and 85%/98% for M. hominis , respectively. Sensitivity for N. gonorrhoeae and U. urealyticum in urethral samples was 100%. Conclusion With good to very good sensitivity depending on the sampling site and pathogen as well as very good specificity overall the eazyplex platform is a useful rapid diagnostic method for POC bacterial STI-testing especially for N. gonorrhoeae and C. trachomatis , allowing for almost immediate treatment initiation.
ISSN:0300-8126
1439-0973
DOI:10.1007/s15010-023-01981-5