Establishment and Application of a Novel In Vitro Model of Microglial Activation in Traumatic Brain Injury

Mechanical impact-induced primary injury after traumatic brain injury (TBI) leads to acute microglial pro-inflammatory activation and consequently mediates neurodegeneration, which is a major secondary brain injury mechanism. However, the detailed pathologic cascades have not been fully elucidated,...

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Bibliographic Details
Published in:The Journal of neuroscience 2023-01, Vol.43 (2), p.319-332
Main Authors: Liu, Ning, Li, Yadan, Jiang, Yinghua, Shi, Samuel, Niamnud, Aim, Vodovoz, Sammy J, Katakam, Prasad V G, Vidoudez, Charles, Dumont, Aaron S, Wang, Xiaoying
Format: Article
Language:English
Subjects:
Animal models
Animals
Brain
Brain Injuries, Traumatic - pathology
Damage patterns
Degeneration
Head injuries
Inflammation
Macrophages - metabolism
Male
Metabolism
Mice
Mice, Inbred C57BL
Microglia
Microglia - metabolism
Neurodegeneration
Neurons - metabolism
Traumatic brain injury
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Summary:Mechanical impact-induced primary injury after traumatic brain injury (TBI) leads to acute microglial pro-inflammatory activation and consequently mediates neurodegeneration, which is a major secondary brain injury mechanism. However, the detailed pathologic cascades have not been fully elucidated, partially because of the pathologic complexity in animal TBI models. Although there are several TBI models, none of them closely mimic post-TBI microglial activation. In the present study, we aimed to establish an TBI model, specifically reconstituting the pro-inflammatory activation and associated neurodegeneration following TBI. We proposed three sets of experiments. First, we established a needle scratch injured neuron-induced microglial activation and neurodegeneration model of TBI. Second, we compared microglial pro-inflammatory cytokines profiles between the TBI model and TBI in male mice. Additionally, we validated the role of injured neurons-derived damage-associated molecular patterns in amplifying microglial pro-inflammatory pathways using the TBI model. Third, we applied the model for the first time to characterize the cellular metabolic profile of needle scratch injured-neuron-activated microglia, and define the role of metabolic reprogramming in mediating pro-inflammatory microglial activation and mediated neurodegeneration. Our results showed that we successfully established a novel TBI model, which closely mimics primary neuronal injury-triggered microglial pro-inflammatory activation and mediated neurodegeneration after TBI. This model provides an advanced and highly translational platform for dissecting interactions in the pathologic processes of neuronal injury-microglial activation-neuronal degeneration cascade, and elucidating the detailed underlying cellular and molecular insights after TBI. Microglial activation is a key component of acute neuroinflammation that leads to neurodegeneration and long-term neurologic outcome deficits after TBI. However, it is not feasible to truly dissect primary neuronal injury-induced microglia activation, and consequently mediated neurodegeneration Furthermore, there is currently lacking of TBI models closely mimicking the TBI primary injury-mediated microglial activation. In this study, we successfully established and validated a novel TBI model of microglial activation, and for the first time, characterized the cellular metabolic profile of microglia in this model. This novel microglial activation TBI mode
ISSN:0270-6474
1529-2401
DOI:10.1523/JNEUROSCI.1539-22.2022