Chlorophyll fluorometry in evaluating photosynthetic performance: key limitations, possibilities, perspectives and alternatives

Non-destructive methods for the assessment of photosynthetic parameters of plants are widely applied to evaluate rapidly the photosynthetic performance, plant health, and shifts in plant productivity induced by environmental and cultivation conditions. Most of these methods are based on measurements...

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Published in:Physiology and molecular biology of plants 2022-12, Vol.28 (11-12), p.2041-2056
Main Authors: Lysenko, Vladimir, D. Rajput, Vishnu, Kumar Singh, Rupesh, Guo, Ya, Kosolapov, Alexey, Usova, Elena, Varduny, Tatyana, Chalenko, Elizaveta, Yadronova, Olga, Dmitriev, Pavel, Zaruba, Tatyana
Format: Article
Language:English
Subjects:
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Cell Biology
Chlorophyll
Electron transport
Fluorescence
Fluorimetry
Fluorometry
Life Sciences
Nondestructive testing
Performance evaluation
Photoacoustics
Photosynthesis
Plant Physiology
Plant Sciences
Plants
Pulse amplitude modulation
Pulse modulation
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Summary:Non-destructive methods for the assessment of photosynthetic parameters of plants are widely applied to evaluate rapidly the photosynthetic performance, plant health, and shifts in plant productivity induced by environmental and cultivation conditions. Most of these methods are based on measurements of chlorophyll fluorescence kinetics, particularly on pulse modulation (PAM) fluorometry. In this paper, fluorescence methods are critically discussed in regard to some their possibilities and limitations inherent to vascular plants and microalgae. Attention is paid to the potential errors related to the underestimation of thylakoidal cyclic electron transport and anoxygenic photosynthesis. PAM-methods are also observed considering the color-addressed measurements. Photoacoustic methods are discussed as an alternative and supplement to fluorometry. Novel Fourier modifications of PAM-fluorometry and photoacoustics are noted as tools allowing simultaneous application of a dual or multi frequency measuring light for one sample.
ISSN:0971-5894
0974-0430
DOI:10.1007/s12298-022-01263-8