Use of oral fluid samples for the investigation of outbreaks of human parvovirus B19 infection

The use of oral fluid (OF) samples for serological diagnosis of parvovirus B19 infection during outbreaks of erythema infectiosum had already been demonstrated, but the feasibility of using OF for the characterization of B19 genotypes circulating during outbreaks has not been described. The aim of t...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2022-12, Vol.53 (4), p.1959-1967
Main Authors: Almada, Daiana Lima, Alves, Arthur Daniel Rocha, Leon, Luciane Almeida Amado, Macedo, Débora Familiar Rodrigues, de Oliveira, Solange Artimos, Siqueira, Marilda Mendonça, Brown, David, Cubel Garcia, Rita de Cássia Nasser
Format: Article
Language:English
Subjects:
Adolescent
Antibodies, Viral
Biomedical and Life Sciences
Clinical Microbiology - Research Paper
Deoxyribonucleic acid
Diagnosis
Disease Outbreaks
DNA
DNA, Viral - analysis
DNA, Viral - genetics
Erythema
Erythema infectiosum
Erythema Infectiosum - diagnosis
Erythema Infectiosum - epidemiology
Food Microbiology
Genetic diversity
Genomes
Genotypes
Genotyping
Humans
Immunoglobulin M
Infections
Life Sciences
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mycology
Nucleotide sequence
Outbreaks
Parvovirus B19, Human - genetics
Parvoviruses
Patients
Polymerase chain reaction
Real-Time Polymerase Chain Reaction
Sequence analysis
Statistical analysis
Strains (organisms)
Citations: Items that this one cites
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Summary:The use of oral fluid (OF) samples for serological diagnosis of parvovirus B19 infection during outbreaks of erythema infectiosum had already been demonstrated, but the feasibility of using OF for the characterization of B19 genotypes circulating during outbreaks has not been described. The aim of this study was to assess the use of “in-house” PCR-based assays as a powerful tool for a rapid diagnosis and molecular characterization of B19 strains in OF samples during outbreaks. Paired serum and OF samples collected from anti-B19 IgM-positive patients, during two outbreaks of ertythema infectiosum (1999–2000 and 2004–2005), were tested by conventional (cPCR) and quantitative PCR (qPCR). qPCR was more sensitive than cPCR for detecting B19-DNA in both OF and serum. Overall, OF presented lower viral load (9.97 × 10 6 UI/mL) than serum (2.42 × 10 10 UI/mL) and this difference was statistically significant. All OF samples obtained from patients in the age group 
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-022-00828-9