Single‐cell RNA‐seq reveals fate determination control of an individual fibre cell initiation in cotton (Gossypium hirsutum)

Summary Cotton fibre is a unicellular seed trichome, and lint fibre initials per seed as a factor determines fibre yield. However, the mechanisms controlling fibre initiation from ovule epidermis are not understood well enough. Here, with single‐cell RNA sequencing (scRNA‐seq), a total of 14 535 cel...

Full description

Saved in:
Bibliographic Details
Published in:Plant biotechnology journal 2022-12, Vol.20 (12), p.2372-2388
Main Authors: Qin, Yuan, Sun, Mengling, Li, Weiwen, Xu, Mingqi, Shao, Lei, Liu, Yuqi, Zhao, Guannan, Liu, Zhenping, Xu, Zhongping, You, Jiaqi, Ye, Zhengxiu, Xu, Jiawen, Yang, Xiyan, Wang, Maojun, Lindsey, Keith, Zhang, Xianlong, Tu, Lili
Format: Article
Language:English
Subjects:
Cell differentiation
cell fate determination
Comparative analysis
Cotton
Cotton Fiber
Cotton fibers
cotton fibre initiation
Crop yield
Developmental stages
Epidermis
Gene expression
Gene sequencing
Genomics
Genotype & phenotype
Gossypium - genetics
Gossypium hirsutum
Hybridization
Integument
Ovule - genetics
regulatory network
Ribonucleic acid
RNA
RNA-Seq
Scanning electron microscopy
single cell transcriptomic atlas
Transcription factors
Trichomes - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Summary Cotton fibre is a unicellular seed trichome, and lint fibre initials per seed as a factor determines fibre yield. However, the mechanisms controlling fibre initiation from ovule epidermis are not understood well enough. Here, with single‐cell RNA sequencing (scRNA‐seq), a total of 14 535 cells were identified from cotton ovule outer integument of Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis and the day of anthesis). Three major cell types, fibre, non‐fibre epidermis and outer pigment layer were identified and then verified by RNA in situ hybridization. A comparative analysis on scRNA‐seq data between Xu142 and its fibreless mutant Xu142 fl further confirmed fibre cluster definition. The developmental trajectory of fibre cell was reconstructed, and fibre cell was identified differentiated at 1 day before anthesis. Gene regulatory networks at four stages revealed the spatiotemporal pattern of core transcription factors, and MYB25‐like and HOX3 were demonstrated played key roles as commanders in fibre differentiation and tip‐biased diffuse growth respectively. A model for early development of a single fibre cell was proposed here, which sheds light on further deciphering mechanism of plant trichome and the improvement of cotton fibre yield. With scRNA‐seq, cotton fibre cell was identified differentiated at −1 DPA. It further refines the spatiotemporal patterns of two command genes, MYB25‐like and HOX3, who determine fibre differentiation and tip‐biased diffuse growth respectively.
ISSN:1467-7644
1467-7652
DOI:10.1111/pbi.13918