Facile synthesis of Cu N-lauroyl sarcosinate nanozymes with laccase-mimicking activity and identification of toxicity effects for C. elegans

Nanozyme is a material with enzyme-like catalytic activity, which has been widely used in environmental, antibacterial, and other fields of research. However, there are few reports on the toxicity of nanozymes. In this work, nanozymes co-assembled from sodium N-lauroyl sarcosinate (Ls) and Cu ions p...

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Bibliographic Details
Published in:RSC advances 2022-11, Vol.12 (51), p.32898-32902
Main Authors: Meng, Fanwei, Ma, Guofang, Qiu, Jiahe, Fu, Zhendong, Yan, Jiaqing, Wang, Lingping
Format: Article
Language:English
Subjects:
Catalytic activity
Chemistry
Electron transfer
Laccase
Nematodes
Physiological effects
Reaction kinetics
Substrates
Toxicity
Trichlorophenols
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Summary:Nanozyme is a material with enzyme-like catalytic activity, which has been widely used in environmental, antibacterial, and other fields of research. However, there are few reports on the toxicity of nanozymes. In this work, nanozymes co-assembled from sodium N-lauroyl sarcosinate (Ls) and Cu ions possess a Cu( i )–Cu( ii ) electron transfer system similar to that of natural laccases. Reaction kinetic studies show that the catalyst follows a typical Michaelis–Menten model. Cu–N-lauroyl sarcosinate nanozyme (Cu-Ls NZ) possess excellent laccase-like activity to oxidize a variety of phenol-containing substrates, such as phenol, 4-iodophenol, and 2,4,5-trichlorophenol. To evaluate the toxicity of the material, the nematode C. elegans was exposed to various concentrations of Cu-Ls NZ. Effects on physiological levels were determined. The results showed that high doses of Cu-Ls NZ increased the amount of reactive oxygen species (ROS), decreased the locomotor activity of nematodes, and inhibited their larval growth.
ISSN:2046-2069
2046-2069
DOI:10.1039/d2ra03759a