Sequential interspecies interactions affect production of antimicrobial secondary metabolites in Pseudomonas protegens DTU9.1

Soil and rhizosphere microbiomes play important roles in suppression of plant pathogens through production of antagonistic secondary metabolites, yet mechanisms that determine the strength of pathogen control are not well understood. Many Pseudomonas species are associated with soil and rhizosphere...

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Bibliographic Details
Published in:The ISME Journal 2022-12, Vol.16 (12), p.2680-2690
Main Authors: Hansen, Morten Lindqvist, Wibowo, Mario, Jarmusch, Scott Alexander, Larsen, Thomas Ostenfeld, Jelsbak, Lars
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - metabolism
Anti-Infective Agents - metabolism
Antibiosis
Antiinfectives and antibacterials
Antimicrobial agents
Biological activity
Biological control
Biosensors
Colonies
Lysis
Metabolites
Microbiomes
Pathogens
Phloroglucinol - metabolism
Phloroglucinol - pharmacology
Pseudomonas
Pseudomonas - metabolism
Rhizosphere
Secondary metabolites
Soil
Soils
Species
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Summary:Soil and rhizosphere microbiomes play important roles in suppression of plant pathogens through production of antagonistic secondary metabolites, yet mechanisms that determine the strength of pathogen control are not well understood. Many Pseudomonas species are associated with soil and rhizosphere microbiomes, and their ability to suppress pathogens is well documented. Here, we investigate how interactions within the Pseudomonas genus affect their production of antimicrobial metabolites. From a biosensor-based screen, we identify P. capeferrum species as capable of modulating secondary metabolite production in P. protegens. We show that P. capeferrum alters production of pyoluteorin and 2,4-diacetylphloroglucinol (DAPG) in P. protegens via two distinct and sequential mechanisms that depends on spatial proximity of the two species. Specifically, P. capeferrum secretes a diffusible signal that induce pyoluteorin production up to 100-fold in neighboring P. protegens colonies. In contrast, the interaction results in reduced DAPG production, but only within mixed-species colonies. Additionally, we found that increased pyoluteorin production and cell lysis of P. capeferrum is required for inhibition of DAPG production, suggesting that pyoluteorin-facilitated antibiosis of P. protegens on P. capeferrum leads to release of cell-associated metabolites and subsequent inhibition of DAPG production in P. protegens. As the interaction modulates in vitro bioactivity of the species, genus-specific interactions may assist in improving efficacy of biocontrol strains and consortia.
ISSN:1751-7362
1751-7370
DOI:10.1038/s41396-022-01322-8