Preserving effect of Loboob (a traditional multi-herbal formulation) on sperm parameters of male rats with busulfan-induced subfertility

Male infertility secondary to exposure to gonadotoxic agents during reproductive age is a concerning issue. The aim of this experimental study was to determine the effect of Loboob on sperm parameters. 55 healthy rats were selected, weighted and divided into five groups consisting of 11 rats each. T...

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Bibliographic Details
Published in:JBRA assisted reproduction 2022-11, Vol.26 (4), p.574-582
Main Authors: Bahmanpour, Soghra, Keshavarz, Mojtaba, Koohpeyma, Farhad, Badr, Parmis, Noori, Adel, Dabbaghmanesh, Mohammad Hossein, Poordast, Tahereh, Najib, Fateme Sadat, Zare, Najaf, Namazi, Niloofar, Jahromi, Bahia Namavar
Format: Article
Language:English
Subjects:
Animals
Busulfan - toxicity
Cancer
Drug dosages
Fatty acids
Honey
Humans
Infertility
Infertility, Male - chemically induced
Infertility, Male - drug therapy
Ingredients
Laboratories
Male
Medicine
Original
Rats
Semen
Sperm
Sperm Count
Sperm Motility
Spermatozoa
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Summary:Male infertility secondary to exposure to gonadotoxic agents during reproductive age is a concerning issue. The aim of this experimental study was to determine the effect of Loboob on sperm parameters. 55 healthy rats were selected, weighted and divided into five groups consisting of 11 rats each. The control group received no medication. Rats in Treatment Group 1 received 10mg/kg Busulfan and rats in Treatment Groups 2, 3, and 4 received 35,70 and 140 mg/kg Loboob respectively in addition to 10mg/kg Busulfan. Finally, the sperm parameters and weights of the rats were compared using the Kolmogorov-Smirnov, non-parametric Kruskal-Wallis, and Dunn-Bonferroni tests. All sperm parameters and weights were significantly decreased among rats receiving Busulfan. All dosages of Loboob were effective to enhance the motility of slow spermatozoa, while only in the rats given 70 and 140 mg/kg of Loboob saw improvements in progressively motile sperm percentages (0.024 and 0.01, respectively). Loboob at a dosage of 140mg/kg improved sperm viability. It did not improve normal morphology sperm or decrease immotile sperm counts. Loboob did not affect mean rat weight. Loboob offered a dose-dependent protective effect on several sperm parameters in rats with busulfan-induced subfertility.
ISSN:1518-0557
1517-5693
1518-0557
DOI:10.5935/1518-0557.20210099