Determination of Manganese in Serum Using GFAAS: Serum Reference Values for Manganese in the Adolescent Girls of the DERVAN Cohort

Current proposed method allows for the determination of manganese in serum sample using aqueous standard calibration technique on Graphite Furnace atomic absorption spectrophotometer (GFAAS) using deuterium background correction. This method involves determination of manganese from digested serum sa...

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Bibliographic Details
Published in:Indian journal of clinical biochemistry 2022-10, Vol.37 (4), p.487-493
Main Authors: Chavan, Sagar, Bhat, Rohit, Nandoskar, Ajit, Bhat, Pallavi, Rokade, Shrawani R., Mohire, Rutuja R., Patil, Suvarna N.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Chemistry/Food Science
Ethylenediaminetetraacetic acid
Life Sciences
Manganese
Microbiology
Original
Original Research Article
Pathology
Pyrolysis
Quantitation
Teenage girls
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Summary:Current proposed method allows for the determination of manganese in serum sample using aqueous standard calibration technique on Graphite Furnace atomic absorption spectrophotometer (GFAAS) using deuterium background correction. This method involves determination of manganese from digested serum samples without the use of matrix modifier. Pyrolysis and atomization temperatures are 1200 °C and 2200 °C respectively. The limit of detection (LoD) and limit of quantitation (LoQ) of the method are 0.0097 ng/ml (0.18 nmol/l) and 0.032 ng/ml (0.58 nmol/l) respectively. Validation of the method was carried out using seronorm trace element level-1 serum standard with excellent agreement between measured value and certified value. Accuracy was demonstrated by the spike and recovery study with analytical recovery between 98.8 and 100.6% in serum. The serum reference value for manganese in adolescent girls of rural Konkan region of India range from 4.7 to 215 nmol/l.
ISSN:0970-1915
0974-0422
DOI:10.1007/s12291-022-01021-3