Bidirectional sequestration between a bacterial hibernation factor and a glutamate metabolizing protein

Bacterial hibernating 100S ribosomes (the 70S dimers) are excluded from translation and are protected from ribonucleolytic degradation, thereby promoting long-term viability and increased regrowth. No extraribosomal target of any hibernation factor has been reported. Here, we discovered a previously...

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Published in:Proceedings of the National Academy of Sciences - PNAS 2022-09, Vol.119 (39), p.e2207257119-e2207257119
Main Authors: Ranava, David, Scheidler, Christopher M, Pfanzelt, Martin, Fiedler, Michaela, Sieber, Stephan A, Schneider, Sabine, Yap, Mee-Ngan F
Format: Article
Language:English
Subjects:
Adenine
Bacteria - metabolism
Binding
Biological Sciences
Glucose - metabolism
Glutamic Acid - metabolism
Hibernation
Humans
Metabolism
NAD
NAD - metabolism
Nicotinamide
Nicotinamide adenine dinucleotide
Oxidoreductases - metabolism
Regrowth
Ribosomal Proteins - metabolism
Ribosomes
Staphylococcus aureus
Staphylococcus aureus - metabolism
Virulence factors
Virulence Factors - metabolism
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Summary:Bacterial hibernating 100S ribosomes (the 70S dimers) are excluded from translation and are protected from ribonucleolytic degradation, thereby promoting long-term viability and increased regrowth. No extraribosomal target of any hibernation factor has been reported. Here, we discovered a previously unrecognized binding partner (YwlG) of hibernation-promoting factor (HPF) in the human pathogen . YwlG is an uncharacterized virulence factor in . We show that the HPF-YwlG interaction is direct, independent of ribosome binding, and functionally linked to cold adaptation and glucose metabolism. Consistent with the distant resemblance of YwlG to the hexameric structures of nicotinamide adenine dinucleotide (NAD)-specific glutamate dehydrogenases (GDHs), YwlG overexpression can compensate for a loss of cellular GDH activity. The reduced abundance of 100S complexes and the suppression of YwlG-dependent GDH activity provide evidence for a two-way sequestration between YwlG and HPF. These findings reveal an unexpected layer of regulation linking the biogenesis of 100S ribosomes to glutamate metabolism.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.2207257119