Assessing the effect of anesthetic gas mixtures on hyperpolarized 13 C pyruvate metabolism in the rat brain

To determine the effect of altering anesthetic oxygen protocols on measurements of cerebral perfusion and metabolism in the rodent brain. Seven rats were anesthetized and underwent serial MRI scans with hyperpolarized [1- C]pyruvate and perfusion weighted imaging. The anesthetic carrier gas protocol...

Full description

Saved in:
Bibliographic Details
Published in:Magnetic resonance in medicine 2022-09, Vol.88 (3), p.1324-1332
Main Authors: Healicon, Richard, Rooney, Catriona H E, Ball, Vicky, Shinozaki, Ayaka, Miller, Jack J, Smart, Sean, Radford-Smith, Daniel, Anthony, Daniel, Tyler, Damian J, Grist, James T
Format: Article
Language:English
Subjects:
Anesthetics, Inhalation - pharmacology
Animals
Bicarbonates - metabolism
Brain - metabolism
Carbon Isotopes - metabolism
Magnetic Resonance Imaging - methods
Oxygen - metabolism
Pyruvic Acid - metabolism
Rats
Technical Note
Technical Note–Preclinical and Clinical Imaging
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To determine the effect of altering anesthetic oxygen protocols on measurements of cerebral perfusion and metabolism in the rodent brain. Seven rats were anesthetized and underwent serial MRI scans with hyperpolarized [1- C]pyruvate and perfusion weighted imaging. The anesthetic carrier gas protocol used varied from 100:0% to 90:10% to 60:40% O :N O. Spectra were quantified with AMARES and perfusion imaging was processed using model-free deconvolution. A 1-way ANOVA was used to compare results across groups, with pairwise t tests performed with correction for multiple comparisons. Spearman's correlation analysis was performed between O % and MR measurements. There was a significant increase in bicarbonate:total C carbon and bicarbonate: C pyruvate when moving between 100:0 to 90:10 and 100:0 to 60:40 O :N O % (0.02 ± 0.01 vs. 0.019 ± 0.005 and 0.02 ± 0.01 vs. 0.05 ± 0.02, respectively) and (0.04 ± 0.01 vs. 0.03 ± 0.01 and 0.04 ± 0.01 vs. 0.08 ± 0.02, respectively). There was a significant difference in C pyruvate time to peak when moving between 100:0 to 90:10 and 100:0 to 60:40 O :N O % (13 ± 2 vs. 10 ± 1 and 13 ± 2 vs. 7.5 ± 0.5 s, respectively) as well as significant differences in cerebral blood flow (CBF) between gas protocols. Significant correlations between bicarbonate: C pyruvate and gas protocol (ρ = -0.47), mean transit time and gas protocol (ρ = 0.41) and C pyruvate time-to-peak and cerebral blood flow (ρ = -0.54) were also observed. These results demonstrate that the detection and quantification of cerebral metabolism and perfusion is dependent on the oxygen protocol used in the anesthetized rodent brain.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.29274