Membrane vesicles from antibiotic‐resistant Staphylococcus aureus transfer antibiotic‐resistance to antibiotic‐susceptible Escherichia coli

Aim Bacteria naturally produce membrane vesicles (MVs), which have been shown to contribute to the spread of multi‐drug resistant bacteria (MDR) by delivering antibiotic‐resistant substances to antibiotic‐susceptible bacteria. Here, we aim to show that MVs from Gram‐positive bacteria are capable of...

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Bibliographic Details
Published in:Journal of applied microbiology 2022-04, Vol.132 (4), p.2746-2759
Main Authors: Lee, Ae Rin, Park, Seong Bin, Kim, Si Won, Jung, Jae Wook, Chun, Jin Hong, Kim, Jaesung, Kim, Young Rim, Lazarte, Jassy Mary S., Jang, Ho Bin, Thompson, Kim D., Jung, Myunghwan, Ha, Min Woo, Jung, Tae Sung
Format: Article
Language:English
Subjects:
Amides
Anti-Bacterial Agents - metabolism
Antibiotics
antibiotic‐resistant bacteria
antibiotic‐susceptible bacteria
Antiinfectives and antibacterials
Bacteria
Coliforms
Drug Resistance, Multiple, Bacterial
E coli
Escherichia coli
Gram-negative bacteria
gram‐positive bacteria
Membrane vesicles
membrane vesicles (MVs)
Membranes
Methicillin
Methicillin-Resistant Staphylococcus aureus
Microbial Sensitivity Tests
Multidrug resistance
Original
Staphylococcus aureus
Vesicles
vesicle‐mediated transferring of antimicrobial resistance
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Summary:Aim Bacteria naturally produce membrane vesicles (MVs), which have been shown to contribute to the spread of multi‐drug resistant bacteria (MDR) by delivering antibiotic‐resistant substances to antibiotic‐susceptible bacteria. Here, we aim to show that MVs from Gram‐positive bacteria are capable of transferring β‐lactam antibiotic‐resistant substances to antibiotic‐sensitive Gram‐negative bacteria. Materials and Methods MVs were collected from a methicillin‐resistant strain of Staphylococcus aureus (MRSA) and vesicle‐mediated fusion with antimicrobial‐sensitive Escherichia coli (RC85). It was performed by exposing the bacteria to the MVs to develop antimicrobial‐resistant E. coli (RC85‐T). Results The RC85‐T exhibited a higher resistance to β‐lactam antibiotics compared to the parent strain. Although the secretion rates of the MVs from RC85‐T and the parent strain were nearly equal, the β‐lactamase activity of the MVs from RC85‐T was 12‐times higher than that of MVs from the parent strain, based on equivalent protein concentrations. Moreover, MVs secreted by RC85‐T were able to protect β‐lactam‐susceptible E. coli from β‐lactam antibiotic‐induced growth inhibition in a dose‐dependent manner. Conclusion MVs play a role in transferring substances from Gram‐positive to Gram‐negative bacteria, shown by the release of MVs from RC85‐T that were able to protect β‐lactam‐susceptible bacteria from β‐lactam antibiotics. Significance and impact of study MVs are involved in the emergence of antibiotic‐resistant strains in a mixed bacterial culture, helping us to understand how the spread of multidrug‐resistant bacteria could be reduced.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.15449