Asymmetric expression of proteins in the granules of the placentomal Binucleate cells in Giraffa camelopardalis

Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (P...

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Bibliographic Details
Published in:Biology of reproduction 2022-04, Vol.106 (4), p.814-822
Main Authors: Wooding, F. B. P, Forhead, A. J, Wilsher, S, Allen, W. R, Roberts, R. M, Green, J. A, Beckers, J. F, Sousa de, N. Melo, Charpigny, G
Format: Article
Language:English
Subjects:
Anatomie (cytologie, histologie, embryologie...) & physiologie
Anatomy (cytology, histology, embryology...) & physiology
Animals
Antibodies
Cell Biology
Female
Fetuses
General Medicine
Giraffa camelopardalis
Giraffe granule protein asymmetry
Giraffes
Glycoproteins
Histology
Immunocytochemistry
Lectins
Lectins - metabolism
Life Sciences
Placenta
Placenta - metabolism
Pregnancy
Prolactin
Prolactin - metabolism
Protein expression
Proteins
Reproductive Biology
Reproductive Medicine
RESEARCH ARTICLE
Ruminant placenta
Ruminants
Ruminants - metabolism
Sciences du vivant
Trophoblast binucleate cell
Trophoblasts
Trophoblasts - metabolism
Villus
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Summary:Mature granulated trophoblast binucleate cells (BNC) have been found in all ruminant placentas examined histologically so far. BNC are normally fairly evenly distributed throughout the fetal villus and all their granules contain a similar variety of hormones and pregnancy associated glycoproteins (PAGs). Only the Giraffe is reported to show a different BNC protein expression, this paper is designed to investigate that. Gold labelled Lectin histochemistry and protein immunocytochemistry were used on deplasticised 1 µm sections of a wide variety of ruminant placentomes with a wide range of antibodies and lectins. In the Giraffe placentomes, even though the lectin histochemistry shows an even distribution of BNC throughout the trophoblast of the placental villi, the protein expression in the BNC granules is limited to the BNC either in the apex or the base of the villi. Placental lactogens and Prolactin (PRL) are present only in basally situated BNC: PAGs only in the apical BNC. PRL is only found in the Giraffe BNC which react with many fewer of the wide range of antibodies used here to investigate the uniformity of protein expression in ruminant BNC. The possible relevance of these differences to ruminant function and evolution is considered to provide a further example of the versatility of the BNC system.
ISSN:0006-3363
1529-7268
1529-7268
DOI:10.1093/biolre/ioab247