Development and validation of a Fast gas chromatography/mass spectrometry method for the determination of cannabinoids in Cannabis sativa L

A routine method for determining cannabinoids in Cannabis sativa L. inflorescence, based on Fast gas chromatography coupled to mass spectrometry (Fast GC/MS), was developed and validated. To avoid the decarboxylation of carboxyl group of cannabinoids, different derivatization approaches, i.e. silyla...

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Bibliographic Details
Published in:Yàowu shi͡p︡in fenxi 2018-10, Vol.26 (4), p.1283-1292
Main Authors: Cardenia, Vladimiro, Gallina Toschi, Tullia, Scappini, Simona, Rubino, Rosamaria Cristina, Rodriguez-Estrada, Maria Teresa
Format: Article
Language:English
Subjects:
Acetic acid
Acids
Cannabinoids
Cannabinoids - chemistry
Cannabis
Cannabis - chemistry
Cannabis sativa
Carboxyl group
Chemical synthesis
Chromatography
Decarboxylation
Diazomethane
Esterification
Ethyl acetate
Fast gas chromatography
Gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Hemp
Marijuana
Mass spectrometry
Mass spectroscopy
Methylation
Molecular Structure
Organic chemistry
Original
Plant Extracts - chemistry
Pyridines
Reagents
Reproducibility
Scientific imaging
Seeds
Sensitivity
Sensitivity and Specificity
Sensors
Spectroscopy
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Summary:A routine method for determining cannabinoids in Cannabis sativa L. inflorescence, based on Fast gas chromatography coupled to mass spectrometry (Fast GC/MS), was developed and validated. To avoid the decarboxylation of carboxyl group of cannabinoids, different derivatization approaches, i.e. silylation and esterification (diazomethane-mediated), reagents and solvents (pyridine or ethyl acetate), were tested. The methylation significantly increased the signal-to-noise ratio of all carboxylic cannabinoids, except for cannabigerolic acid (CBGA). Since diazomethane is not commercially available, is considered a hazardous reactive and requires 1-day synthesis by specialized chemical staff, silylation was used along the whole validation of a routine method. The method gave a fast (total analysis time  1.1), with a good repeatability (intraday 
ISSN:1021-9498
2224-6614
DOI:10.1016/j.jfda.2018.06.001