Anti‐apoptotic effects of human gingival mesenchymal stromal cells on polymorphonuclear leucocytes

Objectives Polymorphonuclear leucocytes (PMNs) constitute the first line of host defence and are crucial in maintaining periodontal health. Their survival and function are modulated by mesenchymal stromal cells (MSCs) from different origin. Gingival MSCs (GMSCs) play an important role in maintaining...

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Published in:Oral diseases 2022-04, Vol.28 (3), p.777-785
Main Authors: Blufstein, Alice, Behm, Christian, Kubin, Barbara, Gahn, Johannes, Moritz, Andreas, Rausch‐Fan, Xiaohui, Andrukhov, Oleh
Format: Article
Language:English
Subjects:
Annexin V
Apoptosis
Cell culture
Coculture Techniques
Culture Media, Conditioned - pharmacology
Flow cytometry
Gingiva
Gum disease
Humans
Immunomodulation
Inflammation
Leukocytes
Mesenchymal Stem Cells
mesenchymal stromal cells
Mesenchyme
Neutrophils
Original
Periodontal Diseases
periodontitis
polymorphonuclear leucocytes
Reactive oxygen species
Stromal cells
Tumor necrosis factor
Tumor necrosis factor-TNF
Tumors
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Summary:Objectives Polymorphonuclear leucocytes (PMNs) constitute the first line of host defence and are crucial in maintaining periodontal health. Their survival and function are modulated by mesenchymal stromal cells (MSCs) from different origin. Gingival MSCs (GMSCs) play an important role in maintaining oral health and in the initial inflammatory response. The present study aimed to investigate the effects of GMSCs on PMNs apoptosis and reactive oxygen species (ROS) production. Methods PMNs were either directly incubated with untreated, interleukin (IL)‐1β‐ or tumour necrosis factor (TNF)‐α‐treated GMSCs or stimulated with their conditioned media. Resulting ROS production was evaluated by dichlorofluorescin diacetate staining, whereas PMNs apoptosis was assessed by Annexin V staining, followed by flow cytometry analysis. Results While conditioned media of untreated and TNF‐α‐treated GMSCs did not affect apoptosis of PMNs, it was significantly delayed by conditioned media of GMSCs treated with IL‐1β. In direct co‐culture, GMSCs exerted anti‐apoptotic effects on PMNs independently of the previous stimulation. However, the strongest impact was observed by IL‐1β‐treated GMSCs. ROS production of PMNs was not influenced by GMSCs or their conditioned media. Conclusion This study demonstrates for the first time the immunomodulatory properties of GMSCs towards PMNs, revealing that IL‐1β enhances anti‐apoptotic effects of GMSCs.
ISSN:1354-523X
1601-0825
DOI:10.1111/odi.13768