Development of 16S rRNA-based probes for the Coriobacterium group and the Atopobium cluster and their application for enumeration of Coriobacteriaceae in human feces from volunteers of different age groups

Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated fr...

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Bibliographic Details
Published in:Applied and environmental microbiology 2000-10, Vol.66 (10), p.4523-4527
Main Authors: HARMSEN, Hermie J. M, WILDEBOER-VELOO, Alida C. M, GRIJPSTRA, Jan, KNOL, Jan, DEGENER, John E, WELLING, Gjalt W
Format: Article
Language:English
Subjects:
Actinobacteria - classification
Actinobacteria - genetics
Actinobacteria - isolation & purification
Adult
Aged
Aged, 80 and over
Aging
Animal, plant and microbial ecology
Biological and medical sciences
Breast Feeding
Child
Child, Preschool
Feces - microbiology
Fundamental and applied biological sciences. Psychology
Humans
In Situ Hybridization, Fluorescence
Infant
Infant Food
Infant, Newborn
Microbial ecology
Middle Aged
Milk, Human
Molecular Sequence Data
Normal microflora of man and animals. Rumen
Phenotype
Phylogeny
Public Health Microbiology
RNA, Ribosomal, 16S - genetics
rRNA 16S
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Summary:Two 16S rRNA-targeted probes were developed: one for the Coriobacterium group and the other for the Atopobium cluster (which comprises most of the Coriobacteriaceae species, including the Coriobacterium group). The new probes were based on sequences of three new Coriobacteriaceae strains isolated from human feces and clinical material and sequences from databases. Application of the probes to fecal samples showed that formula-fed infants had higher numbers of Coriobacterium group cells in their feces than breast-fed infants. In addition, based on the presented results, it is hypothesized that with the increasing age of a person, the diversity of Atopobium cluster species present in the feces increases.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.66.10.4523-4527.2000