Human papillomavirus genotype concordance between Anyplex II HPV28 and linear array HPV genotyping test in anogenital samples

Anyplex II HPV‐28 (HPV‐28) can detect individually 28 HPV genotypes. We assessed the agreement between linear array HPV genotyping (LA‐HPV) and HPV‐28 for detection of 27 HPV genotypes in 410 stored anogenital samples (75 anal samples, 335 physician‐collected cervical samples) collected over 5 years...

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Published in:Journal of medical virology 2022-06, Vol.94 (6), p.2824-2832
Main Authors: Coutlée, François, Pokomandy, Alexandra, Burchell, Ann N., El‐Zein, Mariam, Mayrand, Marie‐Hélène, Rodrigues‐Coutlée, Sophie, Money, Deborah, Comète, Émilie, McClymont, Elisabeth, Rouleau, Danielle, Franco, Eduardo L.
Format: Article
Language:English
Subjects:
Agreements
Alphapapillomavirus - genetics
Anogenital
Cervix Uteri
Confidence intervals
consensus PCR
Deoxyribonucleic acid
DNA
DNA, Viral - genetics
Female
genital cancer
Genital cancers
Genotype
Genotypes
Genotyping
HIV
HPV
Human immunodeficiency virus
Human papillomavirus
Humans
Linear arrays
Male
Papillomaviridae - genetics
Papillomavirus Infections - diagnosis
PCR
Risk
Sensitivity and Specificity
Virology
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Summary:Anyplex II HPV‐28 (HPV‐28) can detect individually 28 HPV genotypes. We assessed the agreement between linear array HPV genotyping (LA‐HPV) and HPV‐28 for detection of 27 HPV genotypes in 410 stored anogenital samples (75 anal samples, 335 physician‐collected cervical samples) collected over 5 years from 410 individuals (13 men, 397 women), including 202 HIV‐seropositive individuals. HPV DNA was detected in 393 (95.9%, 95% confidence interval [CI]: 93.4–97.4) and 382 (93.2%, 95% CI: 90.3–95.3) samples with HPV‐28 and LA‐HPV (p = 0.13), respectively, for a good agreement of 96.3% (κ = 0.65). Of the 10503 HPV typing results, 10195 (780 positive, 9577 negative) were concordant, for an agreement of 97.1% (95% CI: 96.7–97.4) and an excellent of κ = 0.82 (95% CI: 0.80–0.84). The mean type‐specific concordance for 27 genotypes was 97.0%, 95% CI: 95.8–98.5 (κ = 0.86 ± 0.07, 95% CI: 0.83–0.88). Excellent agreement was obtained individually for all high‐risk genotypes (κ = 0.81–0.97) and for most other genotypes except for types 42, 44, 54, 68, and 69. The mean number of types per sample in discordant samples detected with LA‐HPV (3.0, 95% CI: 2.7–3.4) was greater than in concordant samples (1.4, 95% CI: 1.3–1.5; p
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.27605