Potential antimicrobial, antidiabetic, catalytic, antioxidant and ROS/RNS inhibitory activities of Silybum marianum mediated biosynthesized copper oxide nanoparticles

Use of medicinal plants for the biosynthesis of nanoparticles offers several advantages over other synthesis approaches. Plants contain a variety of bioactive compounds that can participate in reduction and capping of nanoparticles. Plant mediated synthesis has the leverage of cost effectiveness, ec...

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Published in:RSC advances 2022-05, Vol.12 (22), p.14069-14083
Main Authors: Iqbal, Junaid, Andleeb, Anisa, Ashraf, Hajra, Meer, Bisma, Mehmood, Azra, Jan, Hasnain, Zaman, Gouhar, Nadeem, Muhammad, Drouet, Samantha, Fazal, Hina, Giglioli-Guivarc'h, Nathalie, Hano, Christophe, Abbasi, Bilal Haider
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antioxidants
Biological properties
Biomedical materials
Biosynthesis
Chemistry
Conjugation
Copper oxides
Cost effectiveness
Enzymes
Fourier transforms
Herbal medicine
High performance liquid chromatography
Life Sciences
Nanoparticles
Photon correlation spectroscopy
Stabilizers (agents)
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Summary:Use of medicinal plants for the biosynthesis of nanoparticles offers several advantages over other synthesis approaches. Plants contain a variety of bioactive compounds that can participate in reduction and capping of nanoparticles. Plant mediated synthesis has the leverage of cost effectiveness, eco-friendly approach and sustained availability. In the current study , a medicinally valuable plant rich in silymarin content, is used as a reducing and stabilizing agent for the fabrication of nanoparticles. Biosynthesized CuO-NPs were characterized using High Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM), and Dynamic Light Scattering (DLS) techniques. Characterization revealed that CuO-NPs having a crystalline structure showed spherical morphology with an average size of 15 nm. HPLC analysis demonstrated conjugation of various silymarin components, especially the presence of silybin A (705.06 ± 1.59 mg g DW). CuO-NPs exhibited strong bactericidal potency against clinically important pathogenic bacterial strains and with an inhibition zone of 18 ± 1.3 mm and 17 ± 1.2 mm, respectively. Synthesized nanoparticles indicated a dose dependent cytotoxic effect against fibroblast cells exhibiting a percentage cell viability of 83.60 ± 1.505% and 55.1 ± 1.80% at 25 μg mL and 100 μg mL concentration, respectively. Moreover, CuO-NPs displayed higher antioxidant potential in terms of (TAC: 96.9 ± 0.26 μg AAE/mg), (TRP: 68.8 ± 0.35 μg AAE/mg), (DPPH: 55.5 ± 0.62%), (ABTS: 332.34 μM) and a significant value for (FRAP: 215.40 μM). Furthermore, enzyme inhibition assays also exhibited excellent enzyme inhibition potential against α-amylase (35.5 ± 1.54%), urease (78.4 ± 1.26%) and lipase (80.50.91%), respectively. Overall findings indicated that biosynthesized CuO-NPs possess immense biological and biomedical properties and could be used as a broad-spectrum agent for a wider range of biomedical applications.
ISSN:2046-2069
2046-2069
DOI:10.1039/d2ra01929a