High-throughput metabolomics enables metabolite biomarkers and metabolic mechanism discovery of fish in response to alkalinity stress

High throughput mass spectrometry (MS)-based metabolomics is a popular platform for small molecule metabolites analyses that are widely used for detecting biomarkers in the research field of environmental assessment. Crucian carp ( , CC) is an economically and ecologically important fish in Asia. It...

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Bibliographic Details
Published in:RSC advances 2018-01, Vol.8 (27), p.14983-14990
Main Authors: Sun, Yan-Chun, Wu, Song, Du, Ning-Ning, Song, Yi, Xu, Wei
Format: Article
Language:English
Subjects:
Adaptation
Alkalinity
Biomarkers
Biosynthesis
Carp
Chemistry
Chemometrics
Degradation
Ecological monitoring
Environmental assessment
Exposure
Fish
Glycine
Long term effects
Mass spectrometry
Metabolism
Metabolites
Methionine
Multivariate statistical analysis
Phenylalanine
Statistical analysis
Stresses
Tyrosine
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Summary:High throughput mass spectrometry (MS)-based metabolomics is a popular platform for small molecule metabolites analyses that are widely used for detecting biomarkers in the research field of environmental assessment. Crucian carp ( , CC) is an economically and ecologically important fish in Asia. It can adapt to extremely high alkalinity, providing us with valuable material to understand the adaptation mechanism for extreme environmental stress. However, the information on the metabolite biomarkers and metabolic mechanisms of CC exposed to alkaline stress is not entirely clear. We applied high-throughput UPLC-Q-TOF/MS combined with chemometrics to identify changes in the metabolome of CC exposed to different concentrations of alkalinity for long term effects. Metabolic differences among alkalinity-treated groups were identified by multivariate statistical analysis. Further, 7 differential metabolites were found after exposure to alkaline conditions. In total, 23 metabolic pathways of these differential metabolites were significantly affected. Alkalinity exposure resulted in widespread change in metabolic profiles in the plasma with disruptions in the phenylalanine metabolism, glycine, serine and threonine metabolism, pyruvate metabolism, tyrosine metabolism, The integrated pathway analysis of the associated metabolites showed that tRNA charging, l-cysteine degradation II, superpathway of methionine degradation, l-serine degradation, tyrosine biosynthesis IV, appear to be the most significantly represented functional categories. Overall, this study demonstrated that metabolic changes in CC played a role in adaptation to the highly alkaline environmental stress.
ISSN:2046-2069
2046-2069
DOI:10.1039/c8ra01317a