Favipiravir (SARS‐CoV‐2) degradation impurities: Identification and route of degradation mechanism in the finished solid dosage form using LC/LC–MS method

Favipiravir finished dosage was approved for emergency use in many countries to treat SARS‐CoV‐2 patients. A specific, accurate, linear, robust, simple, and stability‐indicating HPLC method was developed and validated for the determination of degradation impurities present in favipiravir film‐coated...

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Bibliographic Details
Published in:Biomedical chromatography 2022-06, Vol.36 (6), p.e5363-n/a
Main Authors: Vemuri, Divya Kumar, Gundla, Rambabu, Konduru, Naresh, Mallavarapu, Ravindra, Katari, Naresh Kumar
Format: Article
Language:English
Subjects:
Acetonitriles
Amides
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid - methods
COVID-19
degradation impurities, favipiravir, LC/LC–MS, related substances, SARS‐CoV‐2, stability indicating
Drug Contamination
Drug Stability
Humans
Pyrazines
Reproducibility of Results
SARS-CoV-2
Tandem Mass Spectrometry - methods
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Summary:Favipiravir finished dosage was approved for emergency use in many countries to treat SARS‐CoV‐2 patients. A specific, accurate, linear, robust, simple, and stability‐indicating HPLC method was developed and validated for the determination of degradation impurities present in favipiravir film‐coated tablets. The separation of all impurities was achieved from the stationary phase (Inert sustain AQ‐C18, 250 × 4.6 mm, 5‐μm particle) and mobile phase. Mobile phase A contained KH2PO4 buffer (pH 2.5 ± 0.05) and acetonitrile in the ratio of 98:2 (v/v), and mobile phase B contained water and acetonitrile in the ratio of 50:50 (v/v). The chromatographic conditions were optimized as follows: flow rate, 0.7 mL/min; UV detection, 210 nm; injection volume, 20 μL; and column temperature, 33°C. The proposed method was validated per the current International Conference on Harmonization Q2 (R1) guidelines. The recovery study and linearity ranges were established from the limit of quantification to 150% optimal concentrations. The method validation results were found to be between 98.6 and 106.2% for recovery and r2 = 0.9995–0.9999 for linearity of all identified impurities. The method precision results were achieved below 5% of relative standard deviation. Forced degradation studies were performed in chemical and physical stress conditions. The compound was sensitive to chemical stress conditions. During the study, the analyte degraded and converted to unknown degradation impurities, and its molecular mass was found using the LC–MS technique and established degradation pathways supported by reaction of mechanism. The developed method was found to be suitable for routine analysis of research and development and quality control.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.5363