Thymoquinone modulates the expression of sepsis‑related microRNAs in a CLP model

Sepsis is a clinical syndrome common in critical care settings. In the present study, the therapeutic effect of thymoquinone (TQ) on the expression of sepsis-related microRNAs (miRNAs/miRs), levels of inflammatory markers, organ dysfunction and mortality were investigated in a cecal ligation and pun...

Full description

Saved in:
Bibliographic Details
Published in:Experimental and therapeutic medicine 2022-06, Vol.23 (6), Article 395
Main Authors: Alkharfy, Khalid M, Ahmad, Ajaz, Jan, Basit L, Raish, Mohammad, Rehman, Muneeb U
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Care and treatment
Cytokines
Gene expression
Genetic aspects
Health aspects
Inflammation
MicroRNA
MicroRNAs
Physiological aspects
Quinone
Sepsis
Software
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sepsis is a clinical syndrome common in critical care settings. In the present study, the therapeutic effect of thymoquinone (TQ) on the expression of sepsis-related microRNAs (miRNAs/miRs), levels of inflammatory markers, organ dysfunction and mortality were investigated in a cecal ligation and puncture (CLP) rat model. A single dose of TQ (1 mg/kg) was administered to animals 24 h after CLP and the mortality rate was assessed up to 7 days following the induction of sepsis. In addition, blood samples were collected at different time points and the expression levels of miRNAs (i.e. miR-16, miR-21, miR-27a and miR-34a) were examined, along with the levels of inflammatory cytokines (i.e. TNF-[alpha], IL-1[alpha], IL-2, IL-6 and IL-10) and sepsis markers (i.e. C-reactive protein, endothelial cell-specific molecule-1, VEGF, procalcitonin and D-dimer). Liver, kidney and lung tissues were also collected for further histological examination. Treatment with TQ significantly downregulated the miRNA expression levels, as well as the levels of inflammatory cytokines and early-stage sepsis biomarkers by 30-70% at 12-36 h (P
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2022.11322