Integrin CD11b provides a new marker of pre-germinal center IgA+ B cells in murine Peyer’s patches

Abstract Activated B cells can enter germinal centers (GCs) for affinity maturation to produce high-affinity antibodies. However, which activated B cells will enter GCs remains unknown. Here, we found a small population of CD11b+IgA+ B cells located outside of GCs in murine Peyer’s patches (PPs). Af...

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Bibliographic Details
Published in:International immunology 2022-04, Vol.34 (5), p.249-262
Main Authors: Gao, Peng, Adachi, Takahiro, Okai, Shinsaku, Morita, Naoki, Kitamura, Daisuke, Shinkura, Reiko
Format: Article
Language:English
Subjects:
Animals
CD11b Antigen - immunology
Escherichia coli
Germinal Center
Immunoglobulin A
Integrins - metabolism
Mice
Original Research
Peyer's Patches
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Summary:Abstract Activated B cells can enter germinal centers (GCs) for affinity maturation to produce high-affinity antibodies. However, which activated B cells will enter GCs remains unknown. Here, we found a small population of CD11b+IgA+ B cells located outside of GCs in murine Peyer’s patches (PPs). After injection of the CD11b+IgA+ PP B cells into a PP of a recipient mouse, they entered GCs forty hours later. They expressed GC surface markers and pre-GC B cell genes, suggesting that CD11b provides a novel surface marker of pre-GC IgA+ B cells in murine PPs. Furthermore, independently of dendritic cell activation, CD11b expression on B cells can be induced by bacterial antigens, such as pam3CSK4 and heat-killed Escherichia coli in vitro. In addition, mice orally administered with pam3CSK4 or heat-killed E. coli increased the number of PP GC B cells within two days, and enhanced the mucosal antigen-specific IgA response. Our results demonstrate that the induction of CD11b on B cells is a promising marker for selecting an effective mucosal vaccine adjuvant. A crucial role for CD11b in germinal-center reactions Graphical Abstract Graphical Abstract
ISSN:1460-2377
0953-8178
1460-2377
DOI:10.1093/intimm/dxab113