Screening for polystyrene nanoparticle toxicity on kidneys of adult male albino rats using histopathological, biochemical, and molecular examination results

Polystyrene Nanoparticles (PS-NPs) used for packaging foam, disposable cups, and food containers. Therefore, this study aimed to evaluate PS- NPs toxic effects on kidney of adult male albino rats. A total of 30 rats divided into three groups ( n  = 10): group I negative control group; group II orall...

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Bibliographic Details
Published in:Cell and tissue research 2022-04, Vol.388 (1), p.149-165
Main Authors: Ahmed, Yasmine H., El-Naggar, Mehrez E., Rashad, Maha M., M.Youssef, Ahmed, Galal, Mona K., Bashir, Dina W.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Body weight
Capillaries
Caspase-3
Corpuscles
Creatinine
Cyclooxygenase-2
Cytochrome c
Distal tubules
Glutathione peroxidase
Human Genetics
Kidney - metabolism
Kidney Diseases - metabolism
Kidneys
Male
Malondialdehyde - metabolism
Molecular Medicine
Nanoparticles
Nanoparticles - toxicity
Oral administration
Oxidative Stress
Polystyrene
Polystyrenes - analysis
Polystyrenes - metabolism
Polystyrenes - toxicity
Proteomics
Proximal tubules
Rats
Regular
Regular Article
Renal function
Toxicity
Tubules
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Summary:Polystyrene Nanoparticles (PS-NPs) used for packaging foam, disposable cups, and food containers. Therefore, this study aimed to evaluate PS- NPs toxic effects on kidney of adult male albino rats. A total of 30 rats divided into three groups ( n  = 10): group I negative control group; group II orally administered 3% PS-NPs (3 mg/kg body weight/day) and group III orally administered 3% PS-NPs (10 mg/kg body weight/day) for 35 days. Blood and kidney samples collected and processed for biochemical, histopathological, and immunohistochemical examinations. Results showed that low and high doses PS-NPs had significantly increased serum blood urea nitrogen (BUN), creatinine, malondialdehyde, significantly further reduced glutathione, downregulation of nuclear factor erythroid 2–related factor 2 and glutathione peroxidase, upregulation of caspase-3 and Cytochrome-c. Histopathological examination revealed several alterations. Low dose of PS-NPs exhibited dilated glomerular capillaries, hypotrophy of some renal corpuscles significantly decreases their diameter to 62 μm. Some proximal convoluted tubules and distal convoluted tubules showed loss of cellular architecture with pyknotic nuclei. Hyalinization and vacuolation in renal medulla. In high dose PS-NPs, alterations increased in severity. A significant increase in percentage area of cyclooxygenase-2 in low and high-doses. In conclusion , PS-NPs are a nephrotoxic causing renal dysfunction.
ISSN:0302-766X
1432-0878
DOI:10.1007/s00441-022-03581-5