Eliminating murine norovirus, Helicobacter hepaticus, and intestinal protozoa by embryo transfer for an entire mouse barrier facility

Pathogens can affect physiological and immunological reactions in immunocompromised animals and genetically engineered mice. Specifically, murine norovirus (MNV), Helicobacter, and intestinal protozoa are prevalent in rodent laboratory facilities worldwide. In this study, microbiological test result...

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Bibliographic Details
Published in:Experimental Animals 2022, Vol.71(1), pp.28-35
Main Authors: Kim, Hwan, Bang, Junpil, Baek, Seung Ho, Park, Jae-Hak
Format: Article
Language:English
Subjects:
Bedding
Embryo transfer
Embryos
Entamoeba spp
Genetic engineering
Helicobacter
Helicobacter hepaticus
Immunology
Infections
Intestine
murine norovirus
Original
Pathogens
Protozoa
Rodents
Specific pathogen free
Trichomonas spp
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Summary:Pathogens can affect physiological and immunological reactions in immunocompromised animals and genetically engineered mice. Specifically, murine norovirus (MNV), Helicobacter, and intestinal protozoa are prevalent in rodent laboratory facilities worldwide. In this study, microbiological test results of the soiled bedding of sentinel mice showed the prevalence of MNV (50.9%, 28/55), Helicobacter hepaticus (29.1%, 16/55), Trichomonas spp. (14.5%, 8/55), and Entamoeba spp. (32.7%, 18/55). No single infections were detected as all cases were confirmed to have complex infections with two or four pathogens. In previous studies, the success rate of the cross-fostering method was not perfect; therefore, in this study, the entire mouse strain of the SPF rodent facility was rederived using embryo transfer. For up to three years, we confirmed that the results were negative with regular health surveillance tests. Embryo transfer was, thus, determined to be an effective method for the rederivation of specific pathogen free (SPF) barrier mouse facilities. This is the report for the effectiveness of embryo transfer as an example of successful microbiological clean-up of a mouse colony with multiple infections in an entire SPF mouse facility and embryo transfer may be useful for rederiving.
ISSN:1341-1357
1881-7122
DOI:10.1538/expanim.21-0026