Impacts of lipopolysaccharide on fetal lung developmental maturity and surfactant protein B and surfactant protein C protein expression in gestational diabetes mellitus rats

The rise of bioinformatics based on computer medicine provides a new method to reveal the complex biological data. This experiment is to explore the impacts of lipopolysaccharide on fetal lung developmental maturity and expressions of lung surfactant protein B (SP-B) and lung surfactant protein C (S...

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Bibliographic Details
Published in:Bioengineered 2022-01, Vol.13 (1), p.834-843
Main Authors: Gao, Yue, Zhang, Ziwei, Wang, Yan, Zhou, Dayong, Zhang, Jinghua, Chen, Xiaoyu, Li, Xin, Shao, Qingliang
Format: Article
Language:English
Subjects:
Animals
Body Weight
Case-Control Studies
Diabetes, Gestational - blood
Diabetes, Gestational - genetics
Diabetes, Gestational - metabolism
Disease Models, Animal
Female
Gene Expression Regulation, Developmental - drug effects
Lipopolysaccharides - adverse effects
Lung - drug effects
Lung - embryology
Lung - pathology
Lung - ultrastructure
Male
Peptides - genetics
Peptides - metabolism
Pregnancy
Pulmonary Surfactant-Associated Protein B - genetics
Pulmonary Surfactant-Associated Protein B - metabolism
Random Allocation
Rats
Research Paper
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Summary:The rise of bioinformatics based on computer medicine provides a new method to reveal the complex biological data. This experiment is to explore the impacts of lipopolysaccharide on fetal lung developmental maturity and expressions of lung surfactant protein B (SP-B) and lung surfactant protein C (SP-C) in rats with gestational diabetes mellitus (GDM), thereby discussing the mechanism of developmental disorders in rats. Forty-eight conceived female rats were experimental subjects. Twenty-eight rats were randomly selected to construct the GDM models. All conceived rats underwent section on the 21st day of pregnancy. The ultrastructure of alveolar type II epithelial cells and the morphology of lung tissue were observed under a microscope. The protein localization and expression of SP-B and SP-C were determined by immunohistochemistry; the protein levels of SP-B and SP-C were determined by Western blot. Blood glucose and body weight of the GDM group were higher than those of the control group; the number of alveoli and alveolar area in the GDM group was lower than those in the control group; the alveolar interval in the GDM group was significantly higher than that in the control group (
ISSN:2165-5979
2165-5987
DOI:10.1080/21655979.2021.2013099