Tfap2b specifies an embryonic melanocyte stem cell that retains adult multifate potential

Melanocytes, the pigment-producing cells, are replenished from multiple stem cell niches in adult tissue. Although pigmentation traits are known risk factors for melanoma, we know little about melanocyte stem cell (McSC) populations other than hair follicle McSCs and lack key lineage markers with wh...

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Published in:	Cell reports (Cambridge) 2022-01, Vol.38 (2), p.110234-110234, Article 110234
Main Authors:	Brombin, Alessandro, Simpson, Daniel J., Travnickova, Jana, Brunsdon, Hannah, Zeng, Zhiqiang, Lu, Yuting, Young, Adelaide I.J., Chandra, Tamir, Patton, E. Elizabeth
Format:	Article
Language:	eng
Subjects:	Animals Cell Differentiation - genetics Cell Lineage - genetics chemical genetics ErbB fate mapping melanocyte lineage melanocyte stem cells Melanocytes - metabolism Melanocytes - physiology neural crest Pigmentation - genetics scRNA-seq Skin - metabolism Skin Pigmentation - genetics Stem Cells - classification Stem Cells - metabolism tfap2b Transcription Factor AP-2 - genetics Transcription Factor AP-2 - metabolism zebrafish Zebrafish - genetics Zebrafish Proteins - genetics
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Summary:	Melanocytes, the pigment-producing cells, are replenished from multiple stem cell niches in adult tissue. Although pigmentation traits are known risk factors for melanoma, we know little about melanocyte stem cell (McSC) populations other than hair follicle McSCs and lack key lineage markers with which to identify McSCs and study their function. Here we find that Tfap2b and a select set of target genes specify an McSC population at the dorsal root ganglia in zebrafish. Functionally, Tfap2b is required for only a few late-stage embryonic melanocytes, and is essential for McSC-dependent melanocyte regeneration. Fate mapping data reveal that tfap2b+ McSCs have multifate potential, and are the cells of origin for large patches of adult melanocytes, two other pigment cell types (iridophores and xanthophores), and nerve-associated cells. Hence, Tfap2b confers McSC identity in early development, distinguishing McSCs from other neural crest and pigment cell lineages, and retains multifate potential in the adult zebrafish. [Display omitted] •Tfap2b and its target genes specify McSCs with mixed pigment cell identities•Melanocyte regeneration from McSCs functionally depends on Tfap2b•tfap2b specifies ErbB-dependent McSCs at the stem cell niche•Fate mapping reveals Tfap2b-McSCs have multifate potential for adult pigment cells Brombin et al. use scRNA sequencing to identify the transcriptome of McSCs in zebrafish and discover that tfap2b is a functional marker of this cell type. Fate mapping analyses reveal that these embryonically established stem cells have multifate potential in the adult pattern.
ISSN:	2211-1247 2211-1247