Human adipose tissue–derived mesenchymal stromal cells and their phagocytic capacity

Mesenchymal stromal cells (MSCs) have evidenced considerable therapeutic potential in numerous clinical fields, especially in tissue regeneration. The immunological characteristics of this cell population include the expression of Toll‐like receptors and mannose receptors, among others. The study ob...

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Bibliographic Details
Published in:Journal of cellular and molecular medicine 2022-01, Vol.26 (1), p.178-185
Main Authors: Costela Ruiz, Víctor J., Melguizo Rodríguez, Lucía, Illescas Montes, Rebeca, García Recio, Enrique, Arias Santiago, Salvador, Ruiz, Concepción, De Luna Bertos, Elvira
Format: Article
Language:English
Subjects:
Adipose tissue
Adipose Tissue - cytology
Adipose Tissue - metabolism
Biomedical research
Body fat
Cells, Cultured
Ethics
Experiments
Flow cytometry
Humans
immunogenicity
infection
Latex beads
Mannose
Mannose receptors
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Mesenchyme
Original
Phagocytes
Phagocytes - cytology
Phagocytosis
Severe acute respiratory syndrome coronavirus 2
Software
stem cell‐microenvironment interactions
Stromal cells
Transmission electron microscopy
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Summary:Mesenchymal stromal cells (MSCs) have evidenced considerable therapeutic potential in numerous clinical fields, especially in tissue regeneration. The immunological characteristics of this cell population include the expression of Toll‐like receptors and mannose receptors, among others. The study objective was to determine whether MSCs have phagocytic capacity against different target particles. We isolated and characterized three human adipose tissue MSC (HAT‐MSC) lines from three patients and analysed their phagocytic capacity by flow cytometry, using fluorescent latex beads, and by transmission electron microscopy, using Escherichia coli, Staphylococcus aureus and Candida albicans as biological materials and latex beads as non‐biological material. The results demonstrate that HAT‐MSCs can phagocyte particles of different nature and size. The percentage of phagocytic cells ranged between 33.8% and 56.2% (mean of 44.37% ± 11.253) according to the cell line, and a high phagocytic index was observed. The high phagocytic capacity observed in MSCs, which have known regenerative potential, may offer an advance in the approach to certain local and systemic infections. Study of the phagocytic capacity of human adipose tissue‐derived mesenchymal stromal cells.
ISSN:1582-1838
1582-4934
DOI:10.1111/jcmm.17070