Electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection

This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an impr...

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Bibliographic Details
Published in:Talanta (Oxford) 2022-04, Vol.240, p.123203-123203, Article 123203
Main Authors: Gutiérrez-Gálvez, Laura, del Caño, Rafael, Menéndez-Luque, Iris, García-Nieto, Daniel, Rodríguez-Peña, Micaela, Luna, Mónica, Pineda, Teresa, Pariente, Félix, García-Mendiola, Tania, Lorenzo, Encarnación
Format: Article
Language:English
Subjects:
AuNMs
Biosensing Techniques
COVID-19
DNA
DNA biosensor
Electrochemical Techniques
Gold
Humans
Luminescent Measurements
Metal Nanoparticles
Nanostructures
SARS-CoV-2
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Summary:This work focuses on the development of an electrochemiluminescent nanostructured DNA biosensor for SARS-CoV-2 detection. Gold nanomaterials (AuNMs), specifically, a mixture of gold nanotriangles (AuNTs) and gold nanoparticles (AuNPs), are used to modified disposable electrodes that serve as an improved nanostructured electrochemiluminescent platform for DNA detection. Carbon nanodots (CDs), prepared by green chemistry, are used as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL) and the hybridization is detected by changes in the ECL signal of [Ru(bpy)3]2+/CDs in combination with AuNMs nanostructures. The biosensor is shown to detect a DNA sequence corresponding to SARS-CoV-2 with a detection limit of 514 aM. [Display omitted] •Gold nanomaterials (AuNMs) for nanostructured electrochimioluminiscent platforms development.•DNA biosensor for selective and sensitive SARS-CoV-2 detection.•Carbon nanodots as coreactants agents in the [Ru(bpy)3]2+ anodic electrochemiluminescence (ECL).•Application of the method to directly detect SARS-CoV-2 in human serum samples.
ISSN:0039-9140
1873-3573
DOI:10.1016/j.talanta.2021.123203