Role of Amino Acid Arginine and Nitric Oxide in Mechanisms of Cytoprotective Effect of Non-Opiate Leu-Enkephalin Analogue In Vitro

Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53 + cells (by 40.2%) induced by exposure to H 2 O 2 (60 μM). The cytoprot...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2021-12, Vol.172 (2), p.270-275
Main Authors: Sazonova, E. N., Lebed’ko, O. A., Pinaeva, O. G., Tsimbalist, N. A., Kupriyanova, D. A., Tarasov, P. K., Malofey, Yu. B.
Format: Article
Language:English
Subjects:
Amino acids
Animals
Arginine
Arginine - pharmacology
Arginine - physiology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cells, Cultured
Cytoprotection - drug effects
Enkephalin (leucine)
Enkephalin, Leucine - analogs & derivatives
Enkephalin, Leucine - pharmacology
Enkephalins
Fibroblasts
Fibroblasts - drug effects
Fibroblasts - physiology
Glycine
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Internal Medicine
Laboratory Medicine
Lung - cytology
Narcotics
NG-Nitroarginine methyl ester
NG-Nitroarginine Methyl Ester - pharmacology
Nitric oxide
Nitric Oxide - pharmacology
Nitric Oxide - physiology
Nitric-oxide synthase
Nucleoli
Oxidative stress
Oxidative Stress - drug effects
p53 Protein
Pathology
Peptides
Protective Agents - pharmacology
Rats
Rats, Wistar
Superoxide
Superoxide anions
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Tumor proteins
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Summary:Incubation of primary culture of pulmonary fibroblasts with non-opiate analogue of leuenkephalin (NALE; Phe-D-Ala-Gly-Phe-Leu-Arg, 0.1 μM) reduced generation of superoxide anion-radical (by 20.7%) and decreased the number of p53 + cells (by 40.2%) induced by exposure to H 2 O 2 (60 μM). The cytoprotective effect of NALE was potentiated by NO synthase inhibitor L-NAME (1 mM): the number of p53 + cells decreased by 65.3% and morphometric parameters of the cell nuclei and nucleoli were improved. Incubation of pulmonary fibroblasts culture with peptide G (Phe-D-Ala-Gly-Phe-Leu-Gly, 0.1 μM) also significantly reduced the damaging effect of H 2 O 2 : the number of p53 + cells decreased by 73.5%, the area of cell nuclei returned to normal, and generation of superoxide anion-radical decreased by 18.4%. These results indicate that C-terminal amino acid Arg and activation of NO synthase are not involved in the direct cytoprotective effect of NALE.
ISSN:0007-4888
1573-8221
1573-8221
DOI:10.1007/s10517-021-05374-2