Rapid microwave-assisted biosynthesis of platinum nanoparticles and evaluation of their antioxidant properties and cytotoxic effects against MCF-7 and A549 cell lines

In this study, platinum nanoparticles (Pt NPs) were synthesized by a green method using an aqueous extract of Eucalyptus camaldulensis with assistance of microwave irradiation (850 W) and their physicochemical characteristics were studied by UV–visible spectroscopy, scanning electron microscopy (SEM...

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Bibliographic Details
Published in:3 Biotech 2021-12, Vol.11 (12), p.511, Article 511
Main Authors: Shakibaie, Mojtaba, Torabi-Shamsabad, Reihanehsadat, Forootanfar, Hamid, Amiri-Moghadam, Parinaz, Amirheidari, Bagher, Adeli-Sardou, Mahboubeh, Ameri, Atefeh
Format: Article
Language:English
Subjects:
Agriculture
Anisole
Antioxidants
Bioinformatics
Biomaterials
Biosynthesis
Biotechnology
Cancer Research
Cell death
Cell lines
Cell viability
Chemistry
Chemistry and Materials Science
Cisplatin
Cytotoxicity
Eucalyptus
Evaluation
Fourier transforms
Functional groups
Infrared analysis
Infrared spectroscopy
Nanoparticles
Original
Original Article
Platinum
Scanning electron microscopy
Scavenging
Spectrum analysis
Stem Cells
Toxicity
X-ray diffraction
X-ray spectroscopy
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Summary:In this study, platinum nanoparticles (Pt NPs) were synthesized by a green method using an aqueous extract of Eucalyptus camaldulensis with assistance of microwave irradiation (850 W) and their physicochemical characteristics were studied by UV–visible spectroscopy, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR) analyses. Antioxidant activities, hemocompatibility, and cytotoxic effects of the prepared Pt NPs were then evaluated. The attained results showed that the newly formed Pt NPs possess a size range between 7.4 and 11.2 nm. These spherical-shaped NPs were slightly aggregated and held various functional groups on their surface. The antioxidant activity of Pt nanostructures was comparable to that of butylated hydroxyl anisole at concentrations higher than 320 µg/mL. At the same concentration of 640 μg/mL, the scavenging activities were 3.36 ± 0.9% (hexachloroplatinic acid) and 52.13 ± 0.43% (Pt NPs). The results of hemolytic assay revealed satisfactory hemocompatibility of the Pt NPs even at the concentration as high as 4 mg/mL (hemolysis percent equal to 3.5 ± 1.3%). The cytotoxicity studies revealed that MCF-7, A549, and 3T3 cell lines treated with hexachloroplatinic acid and cisplatin for 24 h and 48 h showed a higher percentage of cell death compared with the Pt NPs. After 24 h, for A549, 3T3, and MCF-7 cells exposed to Pt NPs, the cell viability was measured to be 80 ± 3.2%, 96 ± 1%, and 89 ± 2.6%, respectively, at concentration of 640 µg/mL. Further investigations are required to elucidate the mechanisms behind the biological activities of as-synthesized Pt NPs.
ISSN:2190-572X
2190-5738
DOI:10.1007/s13205-021-03007-z