Intracellular Activity of Antibiotics against Coxiella burnetii in a Model of Activated Human THP-1 Cells

We evaluated antibiotic activity against the intracellular bacterium Coxiella burnetii using an activated THP-1 cell model of infection. At clinically relevant concentrations, the intracellular bacterial load was reduced 300-fold by levofloxacin and finafloxacin, 40-fold by doxycycline, and 4-fold b...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2021-11, Vol.65 (12), p.e0106121-e0106121
Main Authors: Peyrusson, Frédéric, Whelan, Adam O, Hartley, Margaret G, Norville, Isobel H, Harding, Sarah V, Van Bambeke, Françoise
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - therapeutic use
Antimicrobial Chemotherapy
Ciprofloxacin
Coxiella burnetii
Doxycycline - pharmacology
Experimental Therapeutics
Humans
Q Fever - drug therapy
THP-1 Cells
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Summary:We evaluated antibiotic activity against the intracellular bacterium Coxiella burnetii using an activated THP-1 cell model of infection. At clinically relevant concentrations, the intracellular bacterial load was reduced 300-fold by levofloxacin and finafloxacin, 40-fold by doxycycline, and 4-fold by ciprofloxacin and was unaffected by azithromycin. Acidification of the culture medium reduced antibiotic activity, with the exceptions of doxycycline (no change) and finafloxacin (slight improvement). This model may be used to select antibiotics to be evaluated .
ISSN:0066-4804
1098-6596
DOI:10.1128/AAC.01061-21