Role of interleukin-6 in antigen-specific mucosal immunoglobulin A induction by cationic liposomes

•Cationic liposomes elicited IL-6 expression at the site of administration.•Cationic liposomes promoted antigen-specific IL-6 production.•IL-6 played a crucial role in the antigen-specific IgA induction by cationic liposomes. The COVID-19 pandemic, caused by a highly virulent and transmissible patho...

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Bibliographic Details
Published in:International immunopharmacology 2021-12, Vol.101 (Pt A), p.108280-108280, Article 108280
Main Authors: Tada, Rui, Hidaka, Akira, Tanazawa, Yuya, Ohmi, Akari, Muto, Shoko, Ogasawara, Miki, Saito, Momoko, Ohshima, Akihiro, Iwase, Naoko, Honjo, Emi, Kiyono, Hiroshi, Kunisawa, Jun, Negishi, Yoichi
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Antibody Formation - drug effects
Antigens
Antigens - immunology
Cationic liposome
Cations
Cations - immunology
Cations - therapeutic use
COVID-19
COVID-19 - prevention & control
Cytokines
Fatty Acids, Monounsaturated - immunology
Fatty Acids, Monounsaturated - therapeutic use
Female
IgG antibody
Immune response
Immunity, Mucosal - drug effects
Immunity, Mucosal - immunology
Immunization
Immunoglobulin A
Immunoglobulin A - metabolism
Immunoglobulin G
Immunoglobulin G - blood
Immunoglobulins
Infectious diseases
Interleukin 6
Interleukin-6 - antagonists & inhibitors
Interleukin-6 - genetics
Interleukin-6 - immunology
Interleukin-6 - metabolism
Intranasal administration
Liposomes
Liposomes - immunology
Liposomes - therapeutic use
Mice
Mucosal adjuvant
Mucosal immunity
Nasal Mucosa - immunology
Nasal Mucosa - metabolism
Nasal vaccine
Ovalbumin
Ovalbumin - immunology
Pandemics
Public health
Quaternary Ammonium Compounds - immunology
Quaternary Ammonium Compounds - therapeutic use
Spleen - metabolism
Splenocytes
Vaccines
Vaccines - administration & dosage
Vaccines - immunology
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Summary:•Cationic liposomes elicited IL-6 expression at the site of administration.•Cationic liposomes promoted antigen-specific IL-6 production.•IL-6 played a crucial role in the antigen-specific IgA induction by cationic liposomes. The COVID-19 pandemic, caused by a highly virulent and transmissible pathogen, has proven to be devastating to society. Mucosal vaccines that can induce antigen-specific immune responses in both the systemic and mucosal compartments are considered an effective measure to overcome infectious diseases caused by pathogenic microbes. We have recently developed a nasal vaccine system using cationic liposomes composed of 1,2-dioleoyl-3-trimethylammonium-propane and cholesteryl 3β-N-(dimethylaminoethyl)carbamate in mice. However, the comprehensive molecular mechanism(s), especially the host soluble mediator involved in this process, by which cationic liposomes promote antigen-specific mucosal immune responses, remain to be elucidated. Herein, we show that intranasal administration of cationic liposomes elicited interleukin-6 (IL-6) expression at the site of administration. Additionally, both nasal passages and splenocytes from mice nasally immunized with cationic liposomes plus ovalbumin (OVA) were polarized to produce IL-6 when re-stimulated with OVA in vitro. Furthermore, pretreatment with anti-IL-6R antibody, which blocks the biological activities of IL-6, attenuated the production of OVA-specific nasal immunoglobulin A (IgA) but not OVA-specific serum immunoglobulin G (IgG) responses. In this study, we demonstrated that IL-6, exerted by nasally administered cationic liposomes, plays a crucial role in antigen-specific IgA induction.
ISSN:1567-5769
1878-1705
DOI:10.1016/j.intimp.2021.108280