Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay

Direct SARS-CoV-2 Nucleic Acid Detection by Y-Shaped DNA Dual-Probe Transistor Assay

Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the req...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2021-10, Vol.143 (41), p.17004-17014
Main Authors: Kong, Derong, Wang, Xuejun, Gu, Chenjian, Guo, Mingquan, Wang, Yao, Ai, Zhaolin, Zhang, Shen, Chen, Yiheng, Liu, Wentao, Wu, Yungen, Dai, Changhao, Guo, Qianying, Qu, Di, Zhu, Zhaoqin, Xie, Youhua, Liu, Yunqi, Wei, Dacheng
Format: Article
Language:eng
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Summary:Rapid screening of infected individuals from a large population is an effective means in epidemiology, especially to contain outbreaks such as COVID-19. The gold standard assays for COVID-19 diagnostics are mainly based on the reverse transcription polymerase chain reaction, which mismatches the requirements for wide-population screening due to time-consuming nucleic acid extraction and amplification procedures. Here, we report a direct nucleic acid assay by using a graphene field-effect transistor (g-FET) with Y-shaped DNA dual probes (Y-dual probes). The assay relies on Y-dual probes modified on g-FET simultaneously targeting ORF1ab and N genes of SARS-CoV-2 nucleic acid, enabling high a recognition ratio and a limit of detection (0.03 copy μL –1 ) 1–2 orders of magnitude lower than existing nucleic acid assays. The assay realizes the fastest nucleic acid testing (∼1 min) and achieves direct 5-in-1 pooled testing for the first time. Owing to its rapid, ultrasensitive, easily operated features as well as capability in pooled testing, it holds great promise as a comprehensive tool for population-wide screening of COVID-19 and other epidemics.
ISSN:0002-7863
1520-5126