Diet affects glycosylation of serum proteins in women at risk for cardiometabolic disease

Background Glycoproteomics deals with glycoproteins that are formed by post-translational modification when sugars (like fucose and sialic acid) are attached to protein. Glycosylation of proteins influences function, but whether glycosylation is altered by diet is unknown. Objective To evaluate the...

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Bibliographic Details
Published in:European journal of nutrition 2021-10, Vol.60 (7), p.3727-3741
Main Authors: Kim, Tyler, Xie, Yixuan, Li, Qiongyu, Artegoitia, Virginia M., Lebrilla, Carlito B., Keim, Nancy L., Adams, Sean H., Krishnan, Sridevi
Format: Article
Language:English
Subjects:
Blood Proteins - metabolism
Cardiovascular Diseases - prevention & control
Chemistry
Chemistry and Materials Science
Diabetes mellitus (non-insulin dependent)
Diabetes Mellitus, Type 2
Diet
Dietary guidelines
Dietary intake
Female
Glycoproteins
Glycosylation
Humans
Metabolic syndrome
NCT
NCT02298725
Nutrition
Original Contribution
Polysaccharides
Post-translation
Proteins
Serum proteins
Womens health
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Summary:Background Glycoproteomics deals with glycoproteins that are formed by post-translational modification when sugars (like fucose and sialic acid) are attached to protein. Glycosylation of proteins influences function, but whether glycosylation is altered by diet is unknown. Objective To evaluate the effect of consuming a diet based on the Dietary Guidelines for Americans on circulating glycoproteins that have previously been associated with cardiometabolic diseases. Design Forty-four women, with one or more metabolic syndrome characteristics, completed an 8-week randomized controlled feeding intervention ( n  = 22) consuming a diet based on the Dietary Guidelines for Americans (DGA 2010); the remaining consumed a ‘typical American diet’ (TAD, n  = 22). Fasting serum samples were obtained at week0 (baseline) and week8 (post-intervention); 17 serum proteins were chosen for targeted analyses. Protein standards and serum samples were analyzed in a UHPLC-MS protocol to determine peptide concentration and their glycan (fucosylation or sialylation) profiles. Data at baseline were used in correlational analyses; change in proteins and glycans following intervention were used in non-parametric analyses. Results At baseline, women with more metabolic syndrome characteristics had more fucosylation (total di-fucosylated proteins: p  = 0.045) compared to women with a lesser number of metabolic syndrome characteristics. Dietary refined grain intake was associated with increased total fucosylation ( ρ  = − 0.530, p  
ISSN:1436-6207
1436-6215
DOI:10.1007/s00394-021-02539-7