Optimal set of microsatellite markers required to detect illegitimate progenies in selected oil palm (Elaeis guineensis Jacq.) breeding crosses

Oil palm is continually being improved via controlled crossing of selected palms to ensure sustainable yields and productivity. As such, correct parental assignment is important as the presence of illegitimates will compromise the progress of improvement. In the present study, we determined the opti...

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Bibliographic Details
Published in:Breeding Science 2021, Vol.71(2), pp.253-260
Main Authors: Zolkafli, Siti Hazirah, Ithnin, Maizura, Chan, Kuang-Lim, Abidin, Mohd Isa Zainol, Ismail, Ismanizan, Ting, Ngoot Chin, Ooi, Leslie Cheng-Li, Singh, Rajinder
Format: Article
Language:English
Subjects:
Breeding
Deoxyribonucleic acid
DNA
DNA fingerprinting
Elaeis guineensis
Genetic diversity
Genetic fingerprinting
Genetic markers
Germplasm
illegitimacy
Markers
microsatellite markers
Microsatellites
oil palm
Optimization
parentage analysis
Quality control
Research Paper
Sustainable yield
Vegetable oils
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Summary:Oil palm is continually being improved via controlled crossing of selected palms to ensure sustainable yields and productivity. As such, correct parental assignment is important as the presence of illegitimates will compromise the progress of improvement. In the present study, we determined the optimal number of microsatellite (SSR) markers for detection of illegitimates in selected oil palm crosses with high confidence. Determining the optimal number of markers to assign parentage will ensure that the DNA fingerprinting will be cost effective for routine use as a quality control tool in oil palm improvement programs. Here, we evaluated a wide range of crosses that included a cross derived from wild germplasm palm. The results revealed that markers with high PIC are informative and detect most of the alleles present in a cross, including those exhibited by the illegitimates. A larger number of optimum sets of markers are needed to detect all illegitimates for crosses with higher levels of genetic diversity. The optimal number of polymorphic SSR markers determined in the present study can ensure that appropriate quality control is implemented for oil palm improvement programs.
ISSN:1344-7610
1347-3735
DOI:10.1270/jsbbs.19022