Impact of prostatic radiation therapy on bladder contractility and innervation

Aims To determine the effect of prostatic radiation therapy (RT) on bladder contractility and morphology, and axon, or neuron profiles within the detrusor and major pelvic ganglia (MPG) in male rats. Methods Male Sprague‐Dawley rats (8 weeks) received a single dose of prostatic RT (0 or 22 Gy). Blad...

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Published in:Neurourology and urodynamics 2021-08, Vol.40 (6), p.1470-1478
Main Authors: Turner, Alexander C., Powers, Shelby A., Odom, Michael R., Pak, Elena S., Ashcraft, Kathleen A., Koontz, Bridget F., Hannan, Johanna L.
Format: Article
Language:English
Subjects:
Acetyltransferase
Actin
Axons
Bladder
bladder dysfunction
Carbachol
Choline
Choline O-acetyltransferase
Cholinergic nerves
Gene expression
Hydrolase
Innervation
Muscle contraction
Pelvic ganglia
Polymerase chain reaction
Prostate
Prostate cancer
prostatic radiation
Radiation therapy
Smooth muscle
Ubiquitin
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Summary:Aims To determine the effect of prostatic radiation therapy (RT) on bladder contractility and morphology, and axon, or neuron profiles within the detrusor and major pelvic ganglia (MPG) in male rats. Methods Male Sprague‐Dawley rats (8 weeks) received a single dose of prostatic RT (0 or 22 Gy). Bladders and MPG were collected 2‐ and 10‐weeks post‐RT. Detrusor contractile responses to carbachol and electrical field stimulation (EFS) were measured. Bladders were stained with Masson's trichrome, and antibodies for nonspecific neuronal marker, cholinergic nerve marker choline acetyltransferase (ChAT), and alpha‐smooth muscle actin. MPG gene expression was assessed by quantitative polymerase chain reaction for ubiquitin carboxy‐terminal hydrolase L1 (Uchl1) and Chat. Results At 2 weeks post‐RT, bladder smooth muscle, detrusor cholinergic axon profiles, and MPG Chat gene expression were increased (p 
ISSN:0733-2467
1520-6777
DOI:10.1002/nau.24705