Type‐I Interferon assessment in 45 minutes using the FilmArray® PCR platform in SARS‐CoV‐2 and other viral infections

Low concentrations of type‐I interferon (IFN) in blood seem to be associated with more severe forms of Coronavirus disease 2019 (COVID‐19). However, following the type‐I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature o...

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Bibliographic Details
Published in:European Journal of Immunology 2021-04, Vol.51 (4), p.989-994
Main Authors: Mommert, Marine, Perret, Magali, Hockin, Matthew, Viel, Sébastien, Belot, Alexandre, Richard, Jean‐Christophe, Mezidi, Mehdi, Fassier, Jean‐Baptiste, Javouhey, Etienne, Hemmert, Andrew, Mallet, François, Trouillet‐Assant, Sophie, Brengel‐Pesce, Karen
Format: Article
Language:English
Subjects:
Adult
Aged
Child
Clinical application
Clinical trials
Coronaviruses
COVID-19
COVID-19 - blood
COVID-19 - immunology
Female
Fever
FilmArray
Health Personnel
Humans
In vitro diagnostic
Interferon
Interferon Type I - blood
Interferon Type I - genetics
Interferon-alpha - blood
Interferon-alpha - genetics
Life Sciences
Male
Medical personnel
New Technology
Pandemics
Patients
Pediatrics
Polymerase Chain Reaction - methods
SARS-CoV-2 - immunology
Severe acute respiratory syndrome coronavirus 2
Short Communication|Basic
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Summary:Low concentrations of type‐I interferon (IFN) in blood seem to be associated with more severe forms of Coronavirus disease 2019 (COVID‐19). However, following the type‐I interferon response (IR) in early stage disease is a major challenge. We evaluated detection of a molecular interferon signature on a FilmArray® system, which includes PCR assays for four interferon stimulated genes. We analyzed three types of patient populations: (i) children admitted to a pediatric emergency unit for fever and suspected infection, (ii) ICU‐admitted patients with severe COVID‐19, and (iii) healthcare workers with mild COVID‐19. The results were compared to the reference tools, that is, molecular signature assessed with Nanostring® and IFN‐α2 quantification by SIMOA® (Single MOlecule Array). A strong correlation was observed between the IR measured by the FilmArray®, Nanostring®, and SIMOA® platforms (r‐Spearman 0.996 and 0.838, respectively). The FilmArray® panel could be used in the COVID‐19 pandemic to evaluate the IR in 45‐min with 2 min hand‐on‐time at hospitalization and to monitor the IR in future clinical trials. The type‐I Interferon response impairment increases the risk of severe forms of viral infections (like in COVID‐19 disease). We proposed a new integrated tool allowing the fast assessment of type‐I Interferon response (based on interferon stimulated gene expression measurement) to improve patient management.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.202048978