Distribution of Polyphenolic and Isoprenoid Compounds and Biological Activity Differences between in the Fruit Skin + Pulp, Seeds, and Leaves of New Biotypes of Elaeagnus multiflora Thunb

The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of Elaeagnus multiflora Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were de...

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Bibliographic Details
Published in:Antioxidants 2021-06, Vol.10 (6), p.849
Main Authors: Lachowicz-Wiśniewska, Sabina, Kapusta, Ireneusz, Stinco, Carla M, Meléndez-Martínez, Antonio J, Bieniek, Anna, Ochmian, Ireneusz, Gil, Zygmunt
Format: Article
Language:English
Subjects:
Antioxidants
Biological activity
Biotypes
Carotenoids
Chlorophyll
Chromatography
Cultivars
Elaeagnus multiflora
Enzymatic activity
Food
Fruits
Functional foods & nutraceuticals
Gas flow
High-performance liquid chromatography
Leaves
Lycopene
Mass spectrometry
Mass spectroscopy
Organic acids
Pancreas
Polyphenols
Pulp
Scientific imaging
Secondary metabolites
Seeds
Skin
Terpenes
α-Amylase
α-Glucosidase
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Summary:The purpose of this study was to determine the distribution of polyphenolic and isoprenoid compounds and organic acids in the fruit skin + pulp, seeds, and leaves of six new biotypes of Elaeagnus multiflora Thunb., as well as their in vitro biological potency. The polyphenols and isoprenoids were determined with UPLC-PDA-MS/MS (ultra-performance liquid chromatography coupled to photodiode array detection and electrospray ionization tandem mass spectrometry) and RRLC-MS/MS (rapid resolution liquid chromatography/tandem mass spectrometry) methods, the organic acid with HPLC-RID (high-performance liquid chromatography coupled to a Refractive Index Detector), and the antioxidant capacity using ABTS and FRAP assays. Enzymatic activity was established as the ability to inhibit α-amylase, α-glucosidase, and pancreatic lipase. Owing to such an effective technique, 88 compounds were recorded, with 17 polyphenolic compounds and 3 isoprenoids identified for the first time in the seeds and leaves of cherry silverberry. In total, 55 compounds were identified in the leaves, 36 in the seeds, and 31 in the fruit skin + pulp. The predominant polyphenol was polymeric procyanidin (66–95% of total polyphenolics), whereas the predominant isoprenoids were chlorophyll b and (all-E)-lycopene. The results of our work noted that there are significant differences in the profiles of several secondary metabolites between the analyzed parts of the plant, and depending on the need, the compounds can be used to develop different innovative food or cosmetic products.
ISSN:2076-3921
DOI:10.3390/antiox10060849