Susceptibility to AcMNPV and Expression of Recombinant Proteins by a Novel Cell Clone Derived from a Trichoplusia ni QAU-BTI-Tn9-4s Cell Line

It is well known that Tn5B1-4 （commercially known as the High Five） cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell lines. But the characteristics of the cell line do not always remain stable and may change up...

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Bibliographic Details
Published in:Virologica Sinica 2011-10, Vol.26 (5), p.297-305
Main Authors: Shan, Ming, Zhang, Shi-ying, Jiang, Lei, Ma, Ming, Li, Guo-xun
Format: Article
Language:eng ; chi
Subjects:
AcMNPV
Animals
Baculoviridae - growth & development
Baculovirus
Biochemistry
Biomedical and Life Sciences
Biomedicine
BTI
Cell Culture Techniques
Cell Line
Cytology
Genetic Vectors
Growth rate
Infection
Insect cells
insects
Lepidoptera
Medical Microbiology
Microbial Genetics and Genomics
Microbiology
occlusion bodies
Oncology
Polymerase chain reaction
recombinant proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Trichoplusia ni
Virology
viruses
敏感性
昆虫细胞系
杆状病毒
细胞克隆
重组蛋白
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Summary:It is well known that Tn5B1-4 （commercially known as the High Five） cell line is highly susceptible to baculovirus and provides superior production of recombinant proteins when compared to other insect cell lines. But the characteristics of the cell line do not always remain stable and may change upon continuous passage. Recently an alphanodavirus, named Tn5 Cell Line Virus （or TNCL Virus）, was identified in High Five cells in particular. Therefore, we established a new cell line, QB-Tn9-4s, from Trichoplusia ni, which was determined to be free of TNCL virus by RT-PCR analysis. In this paper, we describe the development of a novel cell clone, QB-CL-B, from a low passage QB-Tn9-4s cell line and report its susceptibility to AcMNPV, and the level of recombinant protein production. This cell clone was similar to its parental cells QB-Tn9-4s and Tn5B1-4 cells in morphology and growth rate; although it also showed approximately the same responses to AcMNPV infection and production of occlusion bodies, there were higher levels of recombinant protein production in comparison to QB-Tn9-4s （parental cells） and High5 cells.
ISSN:1674-0769
1995-820X
DOI:10.1007/s12250-011-3201-1