Conserved termini and adjacent variable region of Twortlikevirus Staphylococcus phages

Methicillin-resistant Staphylococcus aureus(MRSA) is an increasing cause of serious infection,both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection are narrowing because of the limited number of newly developed a...

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Bibliographic Details
Published in:Virologica Sinica 2015-12, Vol.30 (6), p.433-440
Main Authors: Zhang, Xianglilan, Kang, Huaixing, Li, Yuyuan, Liu, Xiaodong, Yang, Yu, Li, Shasha, Pei, Guangqian, Sun, Qiang, Shu, Peng, Mi, Zhiqiang, Huang, Yong, Zhang, Zhiyi, Liu, Yannan, An, Xiaoping, Xu, Xiaolu, Tong, Yigang
Format: Article
Language:eng ; chi
Subjects:
antibiotic resistance
bacterial infections
bacteriophages
Base Sequence
Biochemistry
Biomedical and Life Sciences
Biomedicine
clones
DNA, Viral - genetics
Drug Resistance, Bacterial
genome
Genome, Viral
hospitals
host strains
Medical Microbiology
methicillin
Methicillin-Resistant Staphylococcus aureus - virology
Microbial Genetics and Genomics
Microbiology
Microscopy, Electron, Transmission
Oncology
phage
conserved
Phylogeny
Polymerase Chain Reaction - methods
region
Research Article
Sequence Alignment
Sequence Analysis, DNA
Staphylococcus
Staphylococcus aureus
Staphylococcus Phages - genetics
Staphylococcus Phages - isolation & purification
Terminal Repeat Sequences
termini
variable
Twortlikevirus
Virology
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Summary:Methicillin-resistant Staphylococcus aureus(MRSA) is an increasing cause of serious infection,both in the community and hospital settings. Despite sophisticated strategies and efforts, the antibiotic options for treating MRSA infection are narrowing because of the limited number of newly developed antimicrobials. Here, four newly-isolated MRSA-virulent phages, IME-SA1, IMESA2, IME-SA118 and IME-SA119, were sequenced and analyzed. Their genome termini were identified using our previously proposed "termini analysis theory". We provide evidence that remarkable conserved terminus sequences are found in IME-SA1/2/118/119, and, moreover, are widespread throughout Twortlikevirus Staphylococcus phage G1 and K species. Results also suggested that each phage of the two species has conserved 5′ terminus while the 3′ terminus is variable. More importantly, a variable region with a specific pattern was found to be present near the conserved terminus of Twortlikevirus S. phage G1 species. The clone with the longest variable region had variable terminus lengths in successive generations, while the clones with the shortest variable region and with the average length variable region maintained the same terminal length as themselves during successive generations. IME-SA1 bacterial infection experiments showed that the variation is not derived from adaptation of the phage to different host strains. This is the first study of the conserved terminus and variable region of Twortlikevirus S. phages.
ISSN:1674-0769
1995-820X
DOI:10.1007/s12250-015-3643-y