Kinetochore–spindle microtubule interactions during mitosis

The kinetochore is a proteinaceous structure that assembles onto centromeric DNA and mediates chromosome attachment to microtubules during mitosis. This description is deceivingly simple: recent proteomic studies suggest that the diminutive kinetochores of Saccharomyces cerevisiae are comprised of a...

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Bibliographic Details
Published in:Current opinion in cell biology 2005-02, Vol.17 (1), p.35-46
Main Authors: Kline-Smith, Susan L, Sandall, Sharsti, Desai, Arshad
Format: Article
Language:English
Subjects:
Animals
Chromatids - ultrastructure
DNA - metabolism
Histones - metabolism
Humans
Kinetochores - metabolism
Microtubules - metabolism
Microtubules - ultrastructure
Mitosis
Models, Biological
Nuclear Proteins - chemistry
Polymers - chemistry
Saccharomyces cerevisiae
Saccharomyces cerevisiae - physiology
Saccharomyces cerevisiae Proteins - chemistry
Sister Chromatid Exchange
Spindle Apparatus
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Summary:The kinetochore is a proteinaceous structure that assembles onto centromeric DNA and mediates chromosome attachment to microtubules during mitosis. This description is deceivingly simple: recent proteomic studies suggest that the diminutive kinetochores of Saccharomyces cerevisiae are comprised of at least 60 proteins organized into as many as 14 different subcomplexes. Many of these proteins, such as the centromeric histone variant CENP-A, and entire subcomplexes, such as the Ndc80 Hec1 complex, are conserved from yeast to humans despite the diverse nature of the DNA sequences on which they assemble. There have recently been advances in our understanding of the molecular basis of how kinetochores establish dynamic attachments to spindle microtubules, and how these attachments are correctly oriented to ensure segregation of sister chromatids to daughter cells.
ISSN:0955-0674
1879-0410
DOI:10.1016/j.ceb.2004.12.009