Comparative pharmacokinetic analysis of raw and steamed Panax notoginseng roots in rats by UPLC-MS/MS for simultaneously quantifying seven saponins

After being steamed, the restorative effects of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) will be strengthened. However, the underlying mechanism remains elusive. To compare the pharmacokinetics of ginsenosides Rg 1 , Rb 1 , Rd, Re, Rg 5 , Rk 1 , notoginsenoside R 1 (GRg 1 , GRb 1 , GRd, GRe...

Full description

Saved in:
Bibliographic Details
Published in:Pharmaceutical biology 2021-01, Vol.59 (1), p.651-659
Main Authors: Dong, Jiajia, Yin, Zhenzhen, Su, Lianlin, Yu, Mengting, Wang, Meng, Li, Lin, Mao, Chunqin, Lu, Tulin
Format: Article
Language:English
Subjects:
Administration, Oral
Animals
Area Under Curve
Chromatography, High Pressure Liquid - methods
Ginsenosides
Ginsenosides - analysis
Ginsenosides - isolation & purification
Ginsenosides - pharmacokinetics
Male
Oral administration
Panax notoginseng
Panax notoginseng - chemistry
Pharmacokinetics
Plant Extracts - analysis
Plant Extracts - chemistry
Plant Extracts - pharmacokinetics
Rats
Rats, Sprague-Dawley
Reproducibility of Results
Rhodopsin kinase
San-Qi
Saponins
Steam
steam-processed
Tandem Mass Spectrometry
traditional Chinese medicine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:After being steamed, the restorative effects of Panax notoginseng (Burk.) F. H. Chen (Araliaceae) will be strengthened. However, the underlying mechanism remains elusive. To compare the pharmacokinetics of ginsenosides Rg 1 , Rb 1 , Rd, Re, Rg 5 , Rk 1 , notoginsenoside R 1 (GRg 1 , GRb 1 , GRd, GRe, GRg 5 , GRk 1 and NGR 1 ) in the raw and steam-processed P. notoginseng (RPN and SPN). The pharmacokinetics of seven components after oral administration of SPN and RPN extracts (1.0 g/kg) were investigated, respectively, in SD rats (two groups, n = 6) using UPLC-MS/MS. The approach elicited good linear regression (r 2 > 0.991). The accuracy, precision and stability were all within ± 15%. The extraction recoveries and matrix effects were 75.0-100.8% and 85.1-110.3%, respectively. Compared with the RPN group, AUC 0- t of GRg 1 (176.63 ± 42.49 ng/h/mL), GRb 1 (5094.06 ± 1453.14 ng/h/mL), GRd (1396.89 ± 595.14 ng/h/mL), and NGR 1 (135.95 ± 54.32 ng/h/mL), along with C max of GRg 1 (17.41 ± 5.43 ng/mL), GRb 1 (361.48 ± 165.57 ng/mL), GRd (62.47 ± 33.65 ng/mL) and NGR 1 (23.97 ± 16.77 ng/mL) decreased remarkably with oral administration of the SPN extracts, while GRe showed no significantly difference. Of note, GRg 5 and GRk 1 could not be detected in the plasma. Influence of the processing reduced the systemic exposure levels to GRg 1 , GRb 1 , GRd and NGR 1 . It is the first report of comparative pharmacokinetic study of multiple saponins analysis after oral administration of RPN and SPN extract, which might be helpful for further studies on its steam-processing mechanism.
ISSN:1388-0209
1744-5116
DOI:10.1080/13880209.2021.1928239