Phosphatidylethanol in whole blood of rhesus monkeys correlates with ethanol consumption

Background Phosphatidylethanol (PEth) homologs are ethanol metabolites used to identify and monitor alcohol drinking in humans. In this study, we measured levels of the 2 most abundant homologs, PEth 16:0/18:1 and PEth 16:0/18:2, in whole blood samples from rhesus macaque monkeys that drank ethanol...

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Published in:Alcoholism, clinical and experimental research clinical and experimental research, 2021-04, Vol.45 (4), p.689-696
Main Authors: Lopez‐Cruzan, Marisa, Walter, Nicole A. R., Sanchez, Jesus J., Ginsburg, Brett C., Koek, Wouter, Jimenez, Vanessa A., Grant, Kathleen A., Javors, Martin A.
Format: Article
Language:English
Subjects:
Alcohol Drinking - blood
Amino Acid Sequence
Animal models
Animals
blood
Central Nervous System Depressants - administration & dosage
Conserved Sequence
Drinking behavior
Ethanol
Ethanol - administration & dosage
Glycerophospholipids - blood
High-performance liquid chromatography
Humans
Macaca mulatta
Male
Mass spectroscopy
Monkeys & apes
phosphatidylethanol (PEth)
phospholipase D (PLD)
Phospholipase D - chemistry
rhesus monkey
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Summary:Background Phosphatidylethanol (PEth) homologs are ethanol metabolites used to identify and monitor alcohol drinking in humans. In this study, we measured levels of the 2 most abundant homologs, PEth 16:0/18:1 and PEth 16:0/18:2, in whole blood samples from rhesus macaque monkeys that drank ethanol daily ad libitum to assess the relationship between PEth levels and recent ethanol exposure in this animal model. Methods Blood samples were obtained from The Monkey Alcohol Tissue Research Resource. The monkeys were first induced to consume 4% (w/v) ethanol in water from a panel attached to their home cage. Then, monkeys were allowed to drink ethanol and water ad libitum 22 h daily for 12 months and the daily amount of ethanol each monkey consumed was measured. Whole, uncoagulated blood was collected from each animal at the end of the entire experimental procedure. PEth 16:0/18:1 and PEth 16:0/18:2 levels were analyzed by HPLC with tandem mass spectrometry, and the ethanol consumed during the preceding 14 days was measured. Combined PEth was the sum of the concentrations of both homologs. Results Our results show that (1) PEth accumulates in the blood of rhesus monkeys after ethanol consumption; (2) PEth homolog levels were correlated with the daily average ethanol intake during the 14‐day period immediately preceding blood collection; (3) the application of established human PEth 16:0/18:1 cutoff concentrations indicative of light social or no ethanol consumption (
ISSN:0145-6008
1530-0277
1530-0277
DOI:10.1111/acer.14584