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Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance
Tuberculosis (TB) is a disease instigated by Mycobacterium tuberculosis . Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of M. tuberculosis drug resistance i...
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| Published in: | Brazilian journal of microbiology 2021-06, Vol.52 (2), p.607-617 |
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| Main Authors: | , , , , , , , , , |
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| container_end_page | 617 |
| container_issue | 2 |
| container_start_page | 607 |
| container_title | Brazilian journal of microbiology |
| container_volume | 52 |
| creator | Shahzad, Faheem Bashir, Noman Ali, Atia Jabeen, Shagufta Kashif, Mohammad Javaid, Khursheed Tahir, Romeeza Abbas, Afia Jahan, Shah Afzal, Nadeem |
| description | Tuberculosis (TB) is a disease instigated by
Mycobacterium tuberculosis
. Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of
M. tuberculosis
drug resistance in patients with TB. This study was conducted to investigate alterations in monocyte subsets, CD163 expression on monocytes, and its serum level in patients without and with rifampicin resistance TB (RR-TB) and healthy controls. A total of 164 patients with TB (84 without RR-TB and 80 patients with RR-TB) and 85 healthy controls were enrolled in this study. The percentages of various monocyte subsets and surface expression of CD163 on monocytes were quantitatively determined using flow cytometry. The serum level of CD163 was determined by commercially available ELISA kits. Decreased frequency of classical monocytes was detected in patients with RR-TB. Non-classical monocytes were decreased in patients without RR-TB; however, intermediate monocytes were raised in patients with RR-TB. The serum level of CD163 was decreased in patients of RR-TB that showsed a positive correlation with the frequency of CD14
++
CD16
–
CD163
+
and CD14
++
CD16
+
CD163
+
monocytes. It is concluded that decreased classical monocytes and sCD163 in patients with RR-TB could be an indicator of drug resistance. |
| doi_str_mv | 10.1007/s42770-021-00454-x |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8105472</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2523141381</sourcerecordid><originalsourceid>FETCH-LOGICAL-c474t-d3a0c12e450e646e1015f2f3d9faf2fed5fface33303f259242f00316b7a38223</originalsourceid><addsrcrecordid>eNp9kU1P3DAQhq2qqHz-gR4qS730kjL-ShwOlWChgITEBc6W1xlvg7L2Yido-fc1LKWlB05j-X3mnRm9hHxm8J0BNIdZ8qaBCjirAKSS1foD2WF1oyspQX0sb8WaSgvNt8luzncAXIHkn8i2EErX0ModMj9Fl9Bm7KgbbM69swNdxhDd44iZ2tDR2SmrBcX1KmHRY6B9oDcndGXHHsOYjwpUvrrSOcZEo6ddmha0wH0ebXC4T7a8HTIevNQ9cvvz7GZ2UV1dn1_Ojq8qJxs5Vp2w4BhHqQBrWSMDpjz3omu9LRU75b11KIQA4blqueQeQLB63thyIhd75MfGdzXNl9i5slyyg1mlfmnTo4m2N2-V0P8yi_hgNAMlmyeDby8GKd5PmEez7LPDYbAB45QNl7pVrZagC_r1P_QuTimU8wxXXDDJhGaF4hvKpZhzQv-6DAPzFKHZRGhKhOY5QrMuTV_-PeO15U9mBRAbIBcpLDD9nf2O7W_Cjafh</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2523141381</pqid></control><display><type>article</type><title>Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance</title><source>PubMed (Medline)</source><source>Springer Link</source><creator>Shahzad, Faheem ; Bashir, Noman ; Ali, Atia ; Jabeen, Shagufta ; Kashif, Mohammad ; Javaid, Khursheed ; Tahir, Romeeza ; Abbas, Afia ; Jahan, Shah ; Afzal, Nadeem</creator><creatorcontrib>Shahzad, Faheem ; Bashir, Noman ; Ali, Atia ; Jabeen, Shagufta ; Kashif, Mohammad ; Javaid, Khursheed ; Tahir, Romeeza ; Abbas, Afia ; Jahan, Shah ; Afzal, Nadeem</creatorcontrib><description>Tuberculosis (TB) is a disease instigated by
Mycobacterium tuberculosis
. Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of
M. tuberculosis
drug resistance in patients with TB. This study was conducted to investigate alterations in monocyte subsets, CD163 expression on monocytes, and its serum level in patients without and with rifampicin resistance TB (RR-TB) and healthy controls. A total of 164 patients with TB (84 without RR-TB and 80 patients with RR-TB) and 85 healthy controls were enrolled in this study. The percentages of various monocyte subsets and surface expression of CD163 on monocytes were quantitatively determined using flow cytometry. The serum level of CD163 was determined by commercially available ELISA kits. Decreased frequency of classical monocytes was detected in patients with RR-TB. Non-classical monocytes were decreased in patients without RR-TB; however, intermediate monocytes were raised in patients with RR-TB. The serum level of CD163 was decreased in patients of RR-TB that showsed a positive correlation with the frequency of CD14
++
CD16
–
CD163
+
and CD14
++
CD16
+
CD163
+
monocytes. It is concluded that decreased classical monocytes and sCD163 in patients with RR-TB could be an indicator of drug resistance.</description><identifier>ISSN: 1517-8382</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1007/s42770-021-00454-x</identifier><identifier>PMID: 33586094</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Adult ; Antigens, CD - blood ; Antigens, CD - economics ; Antigens, Differentiation, Myelomonocytic - blood ; Antigens, Differentiation, Myelomonocytic - economics ; Antitubercular Agents - pharmacology ; Biomedical and Life Sciences ; CD14 antigen ; CD16 antigen ; CD163 antigen ; Clinical Microbiology - Research Paper ; Drug resistance ; Drug Resistance, Bacterial ; Effector cells ; Female ; Flow cytometry ; Food Microbiology ; Humans ; Innate immunity ; Life Sciences ; Male ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Middle Aged ; Monocytes ; Monocytes - metabolism ; Mycobacterium tuberculosis - drug effects ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - physiology ; Mycology ; Peripheral blood ; Receptors, Cell Surface - blood ; Rifampin ; Rifampin - pharmacology ; Tuberculosis ; Tuberculosis - blood ; Tuberculosis - drug therapy ; Tuberculosis - microbiology</subject><ispartof>Brazilian journal of microbiology, 2021-06, Vol.52 (2), p.607-617</ispartof><rights>Sociedade Brasileira de Microbiologia 2021</rights><rights>Sociedade Brasileira de Microbiologia 2021.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c474t-d3a0c12e450e646e1015f2f3d9faf2fed5fface33303f259242f00316b7a38223</citedby><cites>FETCH-LOGICAL-c474t-d3a0c12e450e646e1015f2f3d9faf2fed5fface33303f259242f00316b7a38223</cites><orcidid>0000-0002-2238-5912</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105472/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105472/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,733,786,790,891,27957,27958,53827,53829</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33586094$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shahzad, Faheem</creatorcontrib><creatorcontrib>Bashir, Noman</creatorcontrib><creatorcontrib>Ali, Atia</creatorcontrib><creatorcontrib>Jabeen, Shagufta</creatorcontrib><creatorcontrib>Kashif, Mohammad</creatorcontrib><creatorcontrib>Javaid, Khursheed</creatorcontrib><creatorcontrib>Tahir, Romeeza</creatorcontrib><creatorcontrib>Abbas, Afia</creatorcontrib><creatorcontrib>Jahan, Shah</creatorcontrib><creatorcontrib>Afzal, Nadeem</creatorcontrib><title>Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><addtitle>Braz J Microbiol</addtitle><description>Tuberculosis (TB) is a disease instigated by
Mycobacterium tuberculosis
. Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of
M. tuberculosis
drug resistance in patients with TB. This study was conducted to investigate alterations in monocyte subsets, CD163 expression on monocytes, and its serum level in patients without and with rifampicin resistance TB (RR-TB) and healthy controls. A total of 164 patients with TB (84 without RR-TB and 80 patients with RR-TB) and 85 healthy controls were enrolled in this study. The percentages of various monocyte subsets and surface expression of CD163 on monocytes were quantitatively determined using flow cytometry. The serum level of CD163 was determined by commercially available ELISA kits. Decreased frequency of classical monocytes was detected in patients with RR-TB. Non-classical monocytes were decreased in patients without RR-TB; however, intermediate monocytes were raised in patients with RR-TB. The serum level of CD163 was decreased in patients of RR-TB that showsed a positive correlation with the frequency of CD14
++
CD16
–
CD163
+
and CD14
++
CD16
+
CD163
+
monocytes. It is concluded that decreased classical monocytes and sCD163 in patients with RR-TB could be an indicator of drug resistance.</description><subject>Adult</subject><subject>Antigens, CD - blood</subject><subject>Antigens, CD - economics</subject><subject>Antigens, Differentiation, Myelomonocytic - blood</subject><subject>Antigens, Differentiation, Myelomonocytic - economics</subject><subject>Antitubercular Agents - pharmacology</subject><subject>Biomedical and Life Sciences</subject><subject>CD14 antigen</subject><subject>CD16 antigen</subject><subject>CD163 antigen</subject><subject>Clinical Microbiology - Research Paper</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>Effector cells</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Food Microbiology</subject><subject>Humans</subject><subject>Innate immunity</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Monocytes</subject><subject>Monocytes - metabolism</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - physiology</subject><subject>Mycology</subject><subject>Peripheral blood</subject><subject>Receptors, Cell Surface - blood</subject><subject>Rifampin</subject><subject>Rifampin - pharmacology</subject><subject>Tuberculosis</subject><subject>Tuberculosis - blood</subject><subject>Tuberculosis - drug therapy</subject><subject>Tuberculosis - microbiology</subject><issn>1517-8382</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kU1P3DAQhq2qqHz-gR4qS730kjL-ShwOlWChgITEBc6W1xlvg7L2Yido-fc1LKWlB05j-X3mnRm9hHxm8J0BNIdZ8qaBCjirAKSS1foD2WF1oyspQX0sb8WaSgvNt8luzncAXIHkn8i2EErX0ModMj9Fl9Bm7KgbbM69swNdxhDd44iZ2tDR2SmrBcX1KmHRY6B9oDcndGXHHsOYjwpUvrrSOcZEo6ddmha0wH0ebXC4T7a8HTIevNQ9cvvz7GZ2UV1dn1_Ojq8qJxs5Vp2w4BhHqQBrWSMDpjz3omu9LRU75b11KIQA4blqueQeQLB63thyIhd75MfGdzXNl9i5slyyg1mlfmnTo4m2N2-V0P8yi_hgNAMlmyeDby8GKd5PmEez7LPDYbAB45QNl7pVrZagC_r1P_QuTimU8wxXXDDJhGaF4hvKpZhzQv-6DAPzFKHZRGhKhOY5QrMuTV_-PeO15U9mBRAbIBcpLDD9nf2O7W_Cjafh</recordid><startdate>20210601</startdate><enddate>20210601</enddate><creator>Shahzad, Faheem</creator><creator>Bashir, Noman</creator><creator>Ali, Atia</creator><creator>Jabeen, Shagufta</creator><creator>Kashif, Mohammad</creator><creator>Javaid, Khursheed</creator><creator>Tahir, Romeeza</creator><creator>Abbas, Afia</creator><creator>Jahan, Shah</creator><creator>Afzal, Nadeem</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0002-2238-5912</orcidid></search><sort><creationdate>20210601</creationdate><title>Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance</title><author>Shahzad, Faheem ; Bashir, Noman ; Ali, Atia ; Jabeen, Shagufta ; Kashif, Mohammad ; Javaid, Khursheed ; Tahir, Romeeza ; Abbas, Afia ; Jahan, Shah ; Afzal, Nadeem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c474t-d3a0c12e450e646e1015f2f3d9faf2fed5fface33303f259242f00316b7a38223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Adult</topic><topic>Antigens, CD - blood</topic><topic>Antigens, CD - economics</topic><topic>Antigens, Differentiation, Myelomonocytic - blood</topic><topic>Antigens, Differentiation, Myelomonocytic - economics</topic><topic>Antitubercular Agents - pharmacology</topic><topic>Biomedical and Life Sciences</topic><topic>CD14 antigen</topic><topic>CD16 antigen</topic><topic>CD163 antigen</topic><topic>Clinical Microbiology - Research Paper</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>Effector cells</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Food Microbiology</topic><topic>Humans</topic><topic>Innate immunity</topic><topic>Life Sciences</topic><topic>Male</topic><topic>Medical Microbiology</topic><topic>Microbial Ecology</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Monocytes</topic><topic>Monocytes - metabolism</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - physiology</topic><topic>Mycology</topic><topic>Peripheral blood</topic><topic>Receptors, Cell Surface - blood</topic><topic>Rifampin</topic><topic>Rifampin - pharmacology</topic><topic>Tuberculosis</topic><topic>Tuberculosis - blood</topic><topic>Tuberculosis - drug therapy</topic><topic>Tuberculosis - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shahzad, Faheem</creatorcontrib><creatorcontrib>Bashir, Noman</creatorcontrib><creatorcontrib>Ali, Atia</creatorcontrib><creatorcontrib>Jabeen, Shagufta</creatorcontrib><creatorcontrib>Kashif, Mohammad</creatorcontrib><creatorcontrib>Javaid, Khursheed</creatorcontrib><creatorcontrib>Tahir, Romeeza</creatorcontrib><creatorcontrib>Abbas, Afia</creatorcontrib><creatorcontrib>Jahan, Shah</creatorcontrib><creatorcontrib>Afzal, Nadeem</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shahzad, Faheem</au><au>Bashir, Noman</au><au>Ali, Atia</au><au>Jabeen, Shagufta</au><au>Kashif, Mohammad</au><au>Javaid, Khursheed</au><au>Tahir, Romeeza</au><au>Abbas, Afia</au><au>Jahan, Shah</au><au>Afzal, Nadeem</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance</atitle><jtitle>Brazilian journal of microbiology</jtitle><stitle>Braz J Microbiol</stitle><addtitle>Braz J Microbiol</addtitle><date>2021-06-01</date><risdate>2021</risdate><volume>52</volume><issue>2</issue><spage>607</spage><epage>617</epage><pages>607-617</pages><issn>1517-8382</issn><eissn>1678-4405</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><notes>Responsible Editor: Fernando R. Pavan</notes><abstract>Tuberculosis (TB) is a disease instigated by
Mycobacterium tuberculosis
. Peripheral blood monocytes represent highly efficient effector cells of innate immunity against TB. Little is known about monocyte subsets and their potential involvement in the development of
M. tuberculosis
drug resistance in patients with TB. This study was conducted to investigate alterations in monocyte subsets, CD163 expression on monocytes, and its serum level in patients without and with rifampicin resistance TB (RR-TB) and healthy controls. A total of 164 patients with TB (84 without RR-TB and 80 patients with RR-TB) and 85 healthy controls were enrolled in this study. The percentages of various monocyte subsets and surface expression of CD163 on monocytes were quantitatively determined using flow cytometry. The serum level of CD163 was determined by commercially available ELISA kits. Decreased frequency of classical monocytes was detected in patients with RR-TB. Non-classical monocytes were decreased in patients without RR-TB; however, intermediate monocytes were raised in patients with RR-TB. The serum level of CD163 was decreased in patients of RR-TB that showsed a positive correlation with the frequency of CD14
++
CD16
–
CD163
+
and CD14
++
CD16
+
CD163
+
monocytes. It is concluded that decreased classical monocytes and sCD163 in patients with RR-TB could be an indicator of drug resistance.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>33586094</pmid><doi>10.1007/s42770-021-00454-x</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2238-5912</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Brazilian journal of microbiology, 2021-06, Vol.52 (2), p.607-617 |
| issn | 1517-8382 1678-4405 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_8105472 |
| source | PubMed (Medline); Springer Link |
| subjects | Adult Antigens, CD - blood Antigens, CD - economics Antigens, Differentiation, Myelomonocytic - blood Antigens, Differentiation, Myelomonocytic - economics Antitubercular Agents - pharmacology Biomedical and Life Sciences CD14 antigen CD16 antigen CD163 antigen Clinical Microbiology - Research Paper Drug resistance Drug Resistance, Bacterial Effector cells Female Flow cytometry Food Microbiology Humans Innate immunity Life Sciences Male Medical Microbiology Microbial Ecology Microbial Genetics and Genomics Microbiology Middle Aged Monocytes Monocytes - metabolism Mycobacterium tuberculosis - drug effects Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - physiology Mycology Peripheral blood Receptors, Cell Surface - blood Rifampin Rifampin - pharmacology Tuberculosis Tuberculosis - blood Tuberculosis - drug therapy Tuberculosis - microbiology |
| title | Decreased classical monocytes and CD163 expression in TB patients: an indicator of drug resistance |
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